Leica Microscope Magnifier DM IRB User Manual

Leica DM IRB

Instructions

Download from Www.Somanuals.com. All Manuals Search And Download.

Leica DM IRB

Instructions

Download from Www.Somanuals.com. All Manuals Search And Download.

Copyrights

All rights to this documentation and the

software it describes are owned by Leica

Microsystems Wetzlar GmbH. Copying of text

and illustrations – in full or in part – by printing,

photostat, microfilm or other techniques, in-

cluding electronic systems, is only permitted

subject to the express written consent of Leica

Microsystems Wetzlar GmbH.

The information contained in the following

documentation represents the latest stage of

technology and knowledge. We have composed

the texts and illustrations with great care.

However, as it is impossible to eliminate the risk

of error completely, we cannot accept any kind

of liability for the correctness of the contents of

this manual. Nevertheless, we are always

grateful to be notified of any errors.

The information in this manual may be altered

without prior notice.

Download from Www.Somanuals.com. All Manuals Search And Download.

Contents

Important notes on this manual .......................

Assembly .............................................................. 17

Assembly tools .................................................... 17

Assembly of the

General safety information ...............................

transmitted light illumination column.............. 17

Intended application .......................................... 10 Assembly of condensers ................................... 18

Assembly of IC condenser prisms ................... 21

The microscope and its components ............. 11 Condenser top ..................................................... 22

Key subassemblies ............................................. 11 Condenser 0.30 S70 ............................................. 22

Stands ................................................................... 12 Condenser 0.53 S23 and 0.90 S1 ....................... 23

Tube mount ........................................................... 12 Assembly of field diaphragm ............................ 24

Tube ..................................................................... 12 Assembly of filters and filter holder ................ 24

Brightness adjustment ....................................... 13 Assembly of ICT objective prisms,

Coarse and fine control ..................................... 13 IC module and IC objective prisms .................. 25

Mains switch ....................................................... 13 Differences between prisms D and D1 ........... 26

Incident light fluorescence device .................. 13 Inserting the analyser/polariser ...................... 26

Aperture diaphragm ........................................... 13 Inserting the fluorescence module ................. 27

Condenser ............................................................ 13 Assembly of the lamp mount,

Condenser height adjustment .......................... 13 mirror housing, lamphousing,

Specimen stages and accessories ................. 14 illumination telescope ........................................ 27

Objective nosepiece and objectives ............... 14 Assembling and exchanging

IR/R tube adapter ................................................ 14 incident light lamps ............................................ 29

Eyepieces ............................................................. 14 Lamphousing 107 L .............................................. 29

Transmitted light illumination unit ................... 14 Lamphousing 106 z L ........................................... 30

Field diaphragm ................................................... 14 Assembling and exchanging

Lamphousings ...................................................... 14 Hg and Xe lamps ................................................. 31

Filters .................................................................... 14 Assembly of the tubes and

tube adapter IR/R ................................................ 33

Installation site ................................................... 15 Adaption of the slide overlay device

and the macro dual system ............................... 36

Unpacking ............................................................ 16 Inserting the eyepieces/graticules ................. 37

Inserting the photoeyepieces ........................... 38

Installation ........................................................... 16 Screwing objectives in and out........................ 38

Assembling the stages, the plane stage

and object guide.................................................. 38

The E version DM IRB/E ..................................... 41

Download from Www.Somanuals.com. All Manuals Search And Download.

Contents

Learn mode .......................................................... 43 Operation

Installing the objective prisms ......................... 43 of transmitted light interference contrast ..... 78

Learning the IC objective prisms ..................... 43

Installing the objectives .................................... 44 Operation of incident light fluorescence ...... 81

Parfocality ............................................................ 47

Dry objectives ...................................................... 47 Operation of filters ............................................. 88

Oil immersion objectives ................................... 47

Exiting the Learn mode ...................................... 47 Operation of the slide overlay device ............ 89

Individual user adjustments .............................. 48

Installing the fluorescence filter cube ........... 49 Operation of the macro device ........................ 90

Concluding the installation ............................... 49

Motorized objective nosepiece........................ 50 Operation of LMC ................................................ 97

Operating modes................................................. 51 Principle of LMC ................................................. 98

DRY and IMMERSION ........................................ 51

Changing the operating mode .......................... 51 Components ......................................................... 99

Automatic lowering of the

objective nosepiece ........................................... 52 Assembly/adjustment ........................................ 100

Brightness adjustment ...................................... 53 Areas of application .......................................... 102

Electronic focus .................................................. 54 Care and maintenance ...................................... 103

Operation .............................................................. 64 Troubleshooting .................................................. 105

Basic setting for transmitted light .................. 64 Storage.................................................................. 112

Operation of objectives ..................................... 70 Packaging and transport................................... 112

Operation of transmitted light.......................... 71 Technical description ........................................ 113

Operation of phase contrast ............................. 74 General technical data...................................... 125

Operation of transmitted light darkfield ........ 76 Main wearing and spare parts, tools ............. 127

Operation of transmitted light polarisation .. 77 EU Conformity declaration ............................... 128

Download from Www.Somanuals.com. All Manuals Search And Download.

Important notes on this manual

This manual is an integral part of the Leica The manual is multi-lingual. Due to the spiral

DM IRB microscope and must be read carefully binding you can turn the language version you

before you start using the microscope.

want to the front.

This manual contains important instructions and The DM IR is available both as a life sciences

information on the operating safety and microscope and as a metallographic/industrial

maintenance of the system. It must therefore be microscope. In cases where the function and

kept in a safe place.

operation are identical, the same text and

illustrations are used in both the separate

instruction manuals.

Text symbols and their meaning:

(1.2)

Numbers in brackets, e. g. (1.2) refer to

illustrations, in this example Fig. 1, item 2.

Numbers with an arrow, e. g. → p. 20 refer to a

particular page in this manual.

→ p. 20

Special safety information is indicated by the

triangular symbol on the left and is given a

grey background.

Caution! Operation errors can damage the

microscope and/or its accessories.

Warning of hot surface.

Explanatory note.

Not part of all configurations.

Download from Www.Somanuals.com. All Manuals Search And Download.

General safety information

This instrument of safety class 1 has been built Make sure that only fuses of the specified type

and tested according to EN 61 010-1/IEC 1010-1/ and rating are used as replacements. It is

VDE 0411-1, safety standards for electrical forbidden to use mended fuses or to short-

measurement, control and laboratory equip- circuit the fuse holder.

ment.

n. b.:

The instruments and accessories described

To keep the microscope in this safe condition,

it is essential to note the advice and warn-

ings given in this manual.

in this manual have been safety-tested and

checked for possible hazards.

Before modifying the instrument in any way

or combining it with non-Leica products not

dealt with in this manual, it is essential to

consult the Leica agency for your area or the

main factory in Wetzlar!

The mains plug must only be inserted into a

grounded outlet.

If an extension cord is used, it must be grounded

as well. Any interruption of the ground

connector inside or outside the microscope or

disconnecting the ground connector can make

the microscope potentially dangerous. Inten-

tional interruption is forbidden!

Any unauthorized alteration to the micro-

scope or use for which it was not intended

will automatically terminate any warranty

claim.

n. b.:

Using the ground connection, any ac-

cessories connected to the microscope

which have their own and/or a different

power supply can be given the same ground

conductor potential. Please consult our

servicing personnel if you intend to connect

units without a ground conductor.

Download from Www.Somanuals.com. All Manuals Search And Download.

n. b.:

The electric accessories of the microscope

are not waterproof. If water gets inside them,

it may cause electrical shock.

Do not put the microscope and its acces-

sories too near a water supply or anywhere

else where water may get inside them.

Protect the microscope from major tem-

perature fluctuations. These may lead to

condensation which can damage the electric

and optical components.

n. b.:

Avoid skin contact when using immersion

oil! Ask the supplier for a safety information

sheet!

Before changing fuses or lamps, always turn

the mains switch off and disconnect the

mains cable.

Download from Www.Somanuals.com. All Manuals Search And Download.

Intended application

The new DM IRB is the logical further

development of the successful inverted

research microscope from Leica. It is used

for examinations of cells and tissue, for

micromanipulation and microinjection tech-

niques all the way through to microdissection or

confocal microscopy. The DM IRB has universal

application potential, incorporating all the

contrasting techniques of brightfield, darkfield,

phase contrast, DIC, fluorescence and

Hoffmann modulation contrast, which are all

easy to use and switch between. Variable

illumination and imaging light paths, HCS optics,

modular accessories and a wide range of

peripherals make the large DM IRB research

microscope from Leica a versatile and powerful

product.

Download from Www.Somanuals.com. All Manuals Search And Download.

The microscope and its components

Key subassemblies

The following views of the whole microscope

show and name important subassemblies of the

microscope and its accessories.

Fig. 1 – 2

1 Binocular phototube, 2 Eyepiece adapter tube, 3 Eyepieces, 4 Tube mount (tube change interface), 5 Tube port for photo/TV

connection, 6 Beamsplitter switch, 7 Mains switch, 8 Brightness adjustment, 9 Lateral TV port, 10 Coaxial coarse and fine

drive, 11 Fluorescence module, 12 ICT prism adjustment, 13 Sextuple objective nosepiece, 14 Centring buttons for incident light

field diaphragm, 15 Field diaphragm adjustment, 16 Filters, 17 Aperture diaphragm adjustment, 18 Lamphousing mount (or

mirrorhousing for two lamphousings), 19 Lamphousing, 20 Stage plate, 21 Analyser, 22 Tube lens module (Bertrand lens and

magnification changer), 23 Switch rod for lateral TV port, 24 Transmitted light illumination column, 25 Condenser, 26 Transmitted

light lamphousing, 27 Transmitted light field diaphragm, 28 SLR port, 29 Second lamphousing

Fig. 1 View from the right

Fig. 2 View from the left

Download from Www.Somanuals.com. All Manuals Search And Download.

The stand with bottom port cannot be equipped

with an SLR front port. This variant is only

produced at a customer’s specific request.

The stand

There are 5 basic versions of the DM IRB stand,

which allow over 50 microscope variants to be

configured.

The electronic stand additionally offers

motorised objective nosepiece, electronic

focusing, IC objective prism coding, LCD display

of microscope functions and (optional)

motorised filter cube changer (RF4-mot module)

with electric dark flap, control panel, etc. All the

above-mentioned stands are also available as

fluorescence stands with integrated fluores-

cence axis. All the fluorescence stands (in-

cluding the manual versions) can be fitted with

the RF4-mot module.

These 5 basic versions are:

– Manual or electronic stand

– With or without integrated fluorescence axis

– With or without SLR front port or bottom port

– Lateral photo port 100 % or 80 %

– With or without integrated magnification

changer

The variants and their components, differences

and uses will be explained individually in this

manual. The function and operation of all

microscopy techniques and the necessary

accessories for the Leica DM IRB will be

described and explained in detail in the Opera-

tion section of this manual.

Tube mount

The interface between the stand and the tube is

called tube mount or tube change interface.

The tube mount is compatible with DM IR tubes

and the IR/R tube adapter which allows the use

of DM R tubes.

First of all, here is a general overview:

Stands

Tube

The basic stand has a photo port on the left for

the adaption of: TV camera, SLR camera or

photomicro system. The variants offered send

either 100 % or 80 % of the light to this photo

port.

The tube, or its tube lens, produces the primary

image together with the objective.

DM IR tubes consist of

basic part, the

binocular part and the tube change ring. The

trinocular tube also has a photo/TV port. A

switchable mirror either directs the light 100 %

to the eyepieces or 100 % to the photo port, or

splits it 50 %/50 %.

Besides the lateral photo port, the SLR stand

also has another port facing the front (front

port*) which can be equipped with either an

SLR camera or a TV camera with c-mount

connection.

Download from Www.Somanuals.com. All Manuals Search And Download.

Brightness adjustment

Aperture diaphragm

A 12 V 100 W transformer is built into the stand The aperture diaphragm determines the

for stepless regulation of brightness with the resolution, field depth and contrast of the

brightness control.

microscope image. The best resolution is

achieved when the apertures of the objectives

and the condenser are roughly the same.

Coarse and fine control

The coarse and fine focus control allows fast

and precise focusing of the microscope image.

Focusing is done by a vertical movement of the

objective nosepiece. The vertical movement

range is 9 mm.

n. b.:

The aperture diaphragm in the illumination

light path is not intended for adjusting the

brightness of the image. This should only be

done by turning the brightness adjustment

knob or using the neutral light damping filter.

Mains switch

The mains switch is used for switching the

microscope power supply on and off.

Incident light fluorescence device

Condenser

The variant with incident light fluorescence

device contains the integrated fluorescence

axis and the lamp mount or a mirrorhousing

for adaption of a second lamphousing. The

fluorescence stand also comprises the fluo-

rescence module which takes 4 filter cubes.

This module is also available as a motorised va-

riant under the name RF4-mot module (see

Technical Description).

The condenser is a lens system through which

the light is collected and focused on the

specimen underneath. The condenser is de-

signed to utilise the numerical aperture of the

objective.

Condenser height adjustment

The markings on the transmitted light illumi-

nation column indicate the height settings of the

different condensers.

Download from Www.Somanuals.com. All Manuals Search And Download.

Specimen stages and accessories

Transmitted light illumination unit

The specimen stage supports the specimens The transmitted light illumination unit consists of

that are to be examined through the mi- the lamphousing and the transmitted light

croscope. Several options are available to illumination column. The transmitted light

accommodate the wide variety of specimens lamphousing comprises a precentred 12 V 100 W

examined, such as object guides, extension halogen lamp and a filter module for three

plates, specimen clips, scanning stage, heating swing-in filters.

stage, etc.

Field diaphragm

Objective nosepiece and objectives

The field diaphragm is used to produce Koehler

The objective nosepiece is used to hold the illumination.

objectives.

objectives with long working

distances, for example, are specially corrected Lamphousings

to respect different thicknesses of vessel bases.

A variety of lamphousings are offered for the

All microscope objectives from magnification

2.5x to 100x can be used. All objectives from the

DM L and DM R range with 25 mm thread are

compatible. The performance data of Leica

objectives can be found in the chapter

“Technical Data; Performance Data” or on the

relevant objective lists available from your Leica

agency.

DM IRB (for halogen, mercury or xenon lamps).

The description and area of application can be

found in the operation section of this manual.

Filters

Filters are generally used to enhance the

contrast of the specimen and are assembled in

the illumination column. A variety of different

filters are easily changed.

IR/R tube adapter

The tube adapter is used to adapt tubes from the

DM R range.

Eyepieces

magnified, virtual image of the actual

intermediate image produced by the objective is

produced with the eyepieces. They act as a

magnifier.

Download from Www.Somanuals.com. All Manuals Search And Download.

Installation site

The microscope should be used in a dust-free

room which is free of oil and chemical fumes

and extreme humidity. Also, the workplace

should not be exposed to major temperature

fluctuations, direct sunlight or vibrations. These

may impair measurements or photographs of the

microscope image.

n. b.:

Lamphousings* and power units* must be

placed at least 10 cm away from the wall and

from flammable objects.

Ambient conditions:

Temperature

10 – 36 °C

Relative humidity

0 – 80 % to 30 °C

Microscopes in warm and humid climates need

special care to prevent build-up of fungus.

Further details are given in the chapters

“Maintenance” and “Storage”.

Download from Www.Somanuals.com. All Manuals Search And Download.

Unpacking

Installation

● First take all the components out of the

transport and packing material.

● Put the basic DM IRB stand on a desk which

has enough room for it.

Please compare the delivery carefully with the

packing note, delivery note or invoice. We

strongly recommend that you keep a copy of

these documents with the manual, so that you

have information on the time and scope of the

delivery later when ordering more equipment or

when the microscope is serviced. Make sure

that no small parts are left in the packing

material. Some of our packing material has

symbols indicating environment-friendly recy-

cling.

n. b.:

On no account should the microscope be

connected to the power socket yet!

n. b.:

Keep the packing material for storage and

transport of the microscope and its accessories.

n. b.:

Try to avoid touching the lens surfaces of the

optics. Any fingermarks on the glass surfaces

should be removed with a soft leather or

linen cloth. Even small amounts of finger

perspiration can attack the surfaces of

optical instruments within a short time. Fur-

ther information is given in the Maintenance

and Cleaning chapters.

Download from Www.Somanuals.com. All Manuals Search And Download.

Assembly

Assembly tools

Assembly of the transmitted light illumination

column

Installation and assembly of the microscope

should preferably be carried out together with a Wipe off the interface surface (4.3) with a dry

member of Leica sales or service staff. cloth. Tilt the illumination column (4.1) slightly to

Only a few ordinary screwdrivers are required the back and insert so that the pin (4.2) engages

for assembly, and these are supplied with the in the groove of the interface surface (4.4).

microscope.

Erect the TL illumination column and secure

with the 4 screws.

When screwing on the TL illumination column,

do not hold onto it so that optimal alignment to

the optical axis is guaranteed. The angle of tilt of

the illumination column can be varied or

clamped securely in the vertical position with

the knurled screw (5.1).

Fig. 3 Assembly tools

1 Cross-tip screwdriver*, 2 Hexagonal screwdriver, 3 mm,

3 Centring keys, 2 mm*, 4 Centring keys, 1.5 mm*, 5 Allen key,

3 mm*, 6 Allen key, 2.5 mm* (short version)

Download from Www.Somanuals.com. All Manuals Search And Download.

The lamphousing for transmitted light Assembly of condensers

illumination for 12 V 100 W halogen lamps with

The technical description of the condensers can

single-lens aspherical collector and heat

protection filter is an integral part of the

transmitted light illumination column. The halo-

gen lamp is preassembled. The chapter on

Troubleshooting includes a description of how

to assemble and change halogen lamps.

be found in the chapter “Technical description”.

All condensers of the Leica DM IRB are

equipped with a 6-position rotating disc (6.2 and

8.3) and can be individually fitted with the

corresponding annular diaphragms for phase

contrast (PH), darkfield (DF) or IC prisms for TL

interference contrast (ICT) (10).

Usually the annular diaphragms are already

inserted in the condenser disc in the factory, so

you will not normally have to fit them yourself.

The condenser disc (11.5) is removed from the

condenser by slackening the screw (11.4) on the

underneath of the condenser.

The cable on the illumination column can then

be connected to the 12 V 100 W socket on the

back of the microscope stand.

Fig. 4 Assembly of transmitted light illumination column

Transmitted light illumination column,

Pin of TL illu-

Fig. 5 Transmitted light illumination column, back view

1 Knurled screw for clamping the transmitted light illumi-

nation column

mination column, 3 Support surface, 4 Groove of support

surface, 5 Drill holes for fixing screws

Download from Www.Somanuals.com. All Manuals Search And Download.

Insert the light rings for Phaco (identified by the – Using the centring keys, screw the centring

code numbers 0, 1, 2, 3 and the intercept

distance S of the corresponding condenser top,

screws back in until they no longer protrude

over the outer edge of the disc.

e. g. 2 S1) and the DF diaphragm (identified by D – Fit IC condenser prisms if appropriate (see

for darkfield and the intercept distance S of the assembly of ICT objective prisms).

corresponding condenser top, e. g. D S1, see – Insert the plastic labels (10.7) in the disc

table “Technical Description”) in the slots of the

disc as follows:

(12.2), allocating them to the corresponding

diaphragms.

– Mark any empty holes with white labels.

– Slightly unscrew the two centring screws

(10.11) using the supplied centring key (12.1).

Insert the disc into the condenser with notches

– Insert the diaphragms so that the mount fits (10.6) facing upwards – towards the aperture

under the spring (10.3) of the slot.

– When the light rings are assembled, their

identification code must be visible i.e. pointing

upwards (12.3, 12.4 and 12.5).

diaphragm (6.3 and 8.4) – and screw down (11.4).

– Insert the light rings in the order 0, 1, 2, 3. The

DF diaphragm can only be inserted in a large

hole.

Fig. 7 Condenser holder

Fig. 6 Condenser 0.30 S70

Condenser slide changer, 2 Condenser centring screws,

Condenser top 0.30 S70 (not for use with condenser base

3 Condenser height adjustment, 4 Condenser clamp screw,

(8.1)), 2 Condenser disc, 3 Aperture diaphragm, 4 Filter holder,

5 Screw for clamping the condenser holder

5 Condenser clamp screw

Download from Www.Somanuals.com. All Manuals Search And Download.

Fig. 8 Condenser 0.53 S23

Condenser base, Condenser top 0.53 S23 (inter-

changeable), 3 Condenser disc, 4 Aperture diaphragm, 5 Filter

holder, 6 Dovetail guide

Fig. 9 Condenser tops for condenser base (8.1)

Condenser top 0.53 S23,

Condenser top 0.90 S1,

3 Condenser top P 1.40 OIL S1, 4 Spacer ring for assembling

9.2 and 9.3

Fig. 10 6-position condenser disc, empty

1 Condenser disc with slots for light rings and IC condenser

prisms, 2 Guide groove for IC condenser prisms (2^ndgroove is

concealed, 3 Spring, 4 Holes for centring keys, 5 Spaces for

label plates,

Notches,

Label plates,

Light ring for

darkfield, 9 IC condenser prism with 2 guide cams, 10 Light

ring for phase contrast, 11 Centring screws

Download from Www.Somanuals.com. All Manuals Search And Download.

– Using the centring keys, screw the centring

screws back in until they no longer protrude

over the outer edge of the disc. The prism is

adjusted with the left centring screw only (see

operation of ICT). The right centring screw

must never restrict the adjustment range.

– Assemble the light rings and DF diaphragm if

appropriate (see previous section).

– Insert the label plates (10.7) corresponding to

the relevant IC condenser prism.

– Mark any empty holes with white labels.

– Remove any finger marks or dust on the

prisms carefully.

Assembly of IC condenser prisms

The IC condenser prisms are assembled at the

factory. The following steps are only necessary

in case of a retrofit:

Remove the condenser disc (11.5) by slackening

the screw (11.4) on the underneath of the

condenser.

– Using the centring keys (12.1), slightly un-

screw the two centring screws (10.11).

– IC condenser prisms can only be inserted into

the large holes of the condenser disc which

have guide grooves (10.2).

– Insert the IC condenser prisms in ascending

order, e. g. K1, K2 and so that the mount fits

under the spring (10.3) in the slot and the

2 guide cams engage in the grooves of the

condenser disc (10.2).

– Put the condenser disc back in the condenser

with the notches (10.6) facing upwards –

towards the aperture diaphragm (6.3 and 8.4).

Screw down the disc (11.4).

– When the prisms are inserted, their

identification code, e. g. K10, must be visible

and pointing towards the centre of the disc

(12.6 and 12.7).

Fig. 12 6-position condenser disc, fully equipped

Fig. 11 Condenser 0.90 S1 (bottom up)

1 Condenser base, 2 Condenser top 0.90 S1, 3 Spacer ring,

4 Fixing screw for condenser disc, 5 Condenser disc

1 Centring keys for centring screws (in working position),

2 Label plates, 3, 4 Light rings for phase contrast, 5 Light ring

for darkfield, 6, 7 IC condenser prisms, 8 Hole for brightfield

Download from Www.Somanuals.com. All Manuals Search And Download.

Condenser top

Assembly of the condensers to the illumination

column

The base and top of condenser 0.30 S70 form a

self-contained unit (Fig. 6).

Condenser 0.30 S70

The condenser top 0.30 S70 (13.4) cannot be

used with the condenser base (8.1).

Tilt the TL illumination column to the back (13.1).

Insert the condenser 0.30 S70 (13.4) from below

into the dovetail guide of the illumination column

(13.2), with the condenser top pointing towards

the microscope stage. Adjust the height of the

condenser until its upper edge is flush with the

condenser height marking S70 on the illumi-

nation column. Secure the condenser with the

supplied hexagonal screwdriver. Erect the TL

illumination column.

The condenser top 0.53 S23 (8.2 and 9.1) is

screwed straight on to the condenser base (8.1).

A spacer ring (9.4 and 11.3) must be used for

assembling the condenser tops 0.90 S1 and

P 1.40 OIL S1 (9.2 and 9.3).

Fig. 13 Assembly of condenser 0.30 S70

Transmitted light illumination column (tilted), 2 Dovetail

guide, Condenser height markings S1, S23 and S70,

Fig. 14 Assembly of condenser 0.30 S70

4 Condenser 0.30 S70, 5 Condenser clamp screw, 6 Field

diaphragm clamp screw, 7 Transmitted light lamphousing

1 Condenser 0.30 S70 in working position (upper edge of

condenser is flush with condenser height marking S70)

Download from Www.Somanuals.com. All Manuals Search And Download.

Condensers 0.53 S23 and 0.90 S1

With the illumination column tilted to the back,

insert the condenser holder (15.4) into the

dovetail guide of the illumination column from

below (15.2). The condenser height adjustment

should point to the left. Adjust the height of the

condenser holder until its upper edge coincides

with the condenser height marking S23 or S1 on

the illumination column (16.1), depending on the

condenser top used. Secure the condenser hold-

er with the hexagonal screwdriver and clamp

Fig. 15 Assembly of condenser holder

1 Transmitted light illumination column, 2 Dovetail guide,

3 Condenser height markings S1, S23 and S70, 4 Condenser

screw (15.5). Mount the base part of the holder, 5 Clamp screw for securing the condenser holder,

6 Clamp screw for field diaphragm module, 7 Transmitted light

lamphousing

condenser with the dovetail guide (8.6) to the

slide change mechanism (7.1) of the condenser

holder (17). The condenser top should point

downwards and the aperture diaphragm control

towards the front (17.3). Slacken the clamp

screw (17.5) and push the condenser back as far

as the stop. Retighten the clamp screw (17.5)

slightly.

Fig. 16 Assembly of condenser holder

Condenser holder in working position for condenser

Fig. 17 Assembly of 0.53 S23 condenser

Dovetail guide of the condenser,

Sliding condenser

0.53 S23 (upper edge of condenser holder coincides with

condenser height marking S23)

changer, 3 Aperture diaphragm adjustment, 4 Condenser top

0.53 S23, 5 Condenser clamp screw

Download from Www.Somanuals.com. All Manuals Search And Download.

Assembly of field diaphragm

To enable Koehler illumination when using The Leica DM IRB is equipped with a holder with

condensers 0.53 S23 and 0.90 S1, field spaces for 3 filters with 40 mm diameter.

Assembly of filters and filter holder

diaphragm has to be assembled. Insert the field The filters are already fitted into the holder at

diaphragm module (18.1) into the mount (Fig. 18) the factory. If you are retrofitting filters yourself,

from below. The diaphragm adjustment (18.2) assemble as follows:

should point in the direction of the tube. Secure

with clamp screw (18.3).

● Slacken the clamp screws (Fig. 19.1) and

remove the filter holder.

● Put the filters into the holder (20).

● Mount the filter holder onto the transmitted

light illumination column and secure in posi-

tion with the clamp screws.

Fig. 18 Assembly of field diaphragm

1 Field diaphragm module, 2 Field diaphragm adjustment,

3 Clamp screw for securing the field diaphragm module

Fig. 19 Assembly of filters

Fig. 20 Assembly of filter holder for 3 filters

1 Clamp screw for securing the filter holder

Download from Www.Somanuals.com. All Manuals Search And Download.

Assembling the ICT objective prisms

Remove the front cover (22.2) under the

objective nosepiece (23.1) after slackening the

Allen screws (22.4).

Insert the IC prism disc (22.1) in the mount and

tighten with the two Allen screws. n. b.: insert

the prism disc with the prism mount pointing

downwards.

Assembling the IC module and IC objective

prisms

The IC prism disc with the IC prisms ordered by

the customer are already assembled in the

microscope at the factory. In case you want to

retrofit the IC prism disc, please proceed as

follows:

Retrofitting individual IC prisms:

Please align prisms against the stop pin (21.4)

and only screw down lightly to avoid strain.

Insert the prisms so that the code letter, e. g. A

points upwards and is readable.

Label the position of the prism on the front of the

ICR prism disc with a label plate (22.5).

Fig. 21 IC objective prism disc without fixing knurl

1 IC objective prism in mount, 2 Code letter (e. g. A), 3 Washer Examples of prisms:

and screw, 4 Stop pin

Prism A – for objectives N PLAN 5x, 10x.

Prisms D and D1 – both for objectives N PLAN

20x, 50x, 100x and HC PL FLUOTAR 5x – 100x.

Abb. 23 Assembly of IC objective prism module

1 Objective nosepiece, 2 Mount for IC objective prism module,

3 Stop pins

Fig. 22 Assembly of IC objective prism module

1 IC objective prism module, 2 Cover, 3 Fixing screw, 4 Hole

for fixing screws, 5 Label plates, 6 Knurled fixing knob

Download from Www.Somanuals.com. All Manuals Search And Download.

Differences between prism D and D1

Inserting the analyser

Prism D is the standard prism with greater Remove the blind slide and insert the analyser

shearing and therefore higher detection sensi- (24.2) from the left as far as the 1^stclickstop.

tivity for minute topological and refractive index

variations in the specimen. Prism D1 has smaller Inserting the polariser

shearing than prism D and a lower detection

The polariser is inserted into the filter holder of

sensitivity for topological and refractive index

the condenser. In addition

whole-wave

variations.

compensator is applied to the back of the

polariser. It is activated by turning the polariser

over, in order to enable colour contrasting in

polarisation or interference contrast (the com-

pensator is active when the lambda symbol λ is

visible from above).

However, prism D1 is better at resolving details

of fine specimen structures.

Fig. 24 Polariser/analyser

Fig. 25 Condenser 0.53 S23

1 Polariser POL , 2 Analyser ICT ↔

1 Filter holder with polariser inserted (swung out)

Download from Www.Somanuals.com. All Manuals Search And Download.

Inserting the fluorescence module

Assembly of the lamp mount, mirror housing,

lamphousing, illumination telescope

The fluorescence module (Fig. 26) is part of the

fluorescence stand, but is also available as a 1. Insert the lamp mount or mirror housing in

retrofit kit. To retrofit the fluorescence module,

remove the blind cover from the stand. The

fluorescence module can be fitted with up to

four different filter cubes (26.3). They are

the back panel and screw down with Allen

screws. Engage the guide pin of the lamp

mount (29.1) in the back panel of the

microscope stand (28.2).

inserted into the dovetail mount (26.2) of the 2. Hold the lamphousings 107/2, 107, 106 z

fluorescence module with their engraving facing

downwards (towards the turret plate). The

against the lamphousing mount and secure

with the fixing screw (Fig. 31).

fluorescence module is inserted on a dovetail 3. We recommend using the illumination

guide into its slot on the stand by pushing it as

far as the stop. One part of the fluorescence

module is the anti-glare protection (27.1), which

can be inserted between the tube and the stage.

Proceed in the same way if you are inserting a

motorised filter cube changer instead of the

manual filter module. Also read the manual for

the electronic version.

telescope for gas discharge lamps. This is

inserted between the lamp mount and the

lamphousing 106 z (30.4) and magnifies the

image of the focal point of the lamp by the

factor 2x in the entrance pupil of the

objective. This results in

significantly

higher illumination intensity for fluorescence.

Fig. 26 Fluorescence module

1 Rotatable turret, 2, 4 Dovetail mounts for filter cubes (the

numbers 1 – 4 are markings of the assembly positions),

3 Filter cubes, 4 Display of the position in the light path,

5 Switch rod with BG 38 and light stop

Fig. 27 Anti-glare protection

Download from Www.Somanuals.com. All Manuals Search And Download.

4. Connect the lamp plug to the connecting

socket in the stand (28.3).

5. Insert light filters, 50 mm Ø into the 2 spaces

in the lamphousing mounts (29.4).

n. b.:

Connect the appliance cable to the mains

socket on the microscope stand (28.4)!

Fig. 28 Back view of microscope

Fig. 29 Lamp mount

Space for assembling

lamphousing mount or mirror

1 Guide pin, 2 Lateral lamphousing mount, 3 Dovetail ring for

mounting to stand, 4 2 spaces for light filters, 5 Allen screws

for fixing

housing, 2 Hole for guide pin, 3 Socket for lamp plug, 4 Mains

socket, 5 Potential equalisation

Fig. 30 Mirror housing and illumination telescope

Fig. 31 Lamphousing 106 z L

1 Lever for mirror switching, 2 Lateral lamphousing mount

with fixing screw, 3 Back lamphousing mount with Allen

screw, 4 Illumination telescope for gas discharge lamps

1 Collector adjustment, 2 Vertical lamp adjustment, 3 Horizon-

tal lamp adjustment, 4 Mount ring

Download from Www.Somanuals.com. All Manuals Search And Download.

Assembling and exchanging incident light

lamps

Lamphousing 107 L

Slacken the fixing screw on the cover and lift off

the cover (Fig. 32a and 32b). Move the collector

to the front and pull the defect 12 V 100 W lamp

out of the base towards the front. Without

removing its protective cover, put a new lamp

into the base, without tilting, as far as it will go.

Exchanging the 12 V 100 W halogen lamp:

n. b.:

Disconnect the lamp and lamphousing from

the power supply. Pull out the mains plug.

n. b.:

Leave the protective cover on the lamp until it

is in position.

Avoid making finger marks or wipe off

immediately. Close the lamphousing.

Fig. 32a – c Lamphousing 107 L

1 Mount with halogen lamp, 2 Collector

Download from Www.Somanuals.com. All Manuals Search And Download.

Lamphousing 106 z L

n. b.:

Slacken the fixing screw on the lid (33.10). Pull

the cut-out plug slightly out of the socket and flip

up lid (33.11; 33.1).

It is important to leave the protective cover

on the lamp until it is in position.

Avoid making finger marks or wipe off

immediately. Close the lamphousing.

Move the collector to the front and lift the defect

lamp out of the base (33.1; 33.2; 33.3). For

convenience, the lamp holder can be removed

from the lamphousing as well. To do this,

slacken the fixing screws on the lamp holder

(33.10) and pull out lamp holder (Fig. 34).

Without removing its protective cover, put a new

lamp into the base, without tilting, as far as it will

go.

Fig. 33 Lamphousing 106 z L

1 Lid, flipped up, 2 Collector, 3 12 V 100 W halogen lamp or gas

discharge lamp in holder, 4, 9 Cover fixing screws, 5 Reflector,

6, 7, 8 x-y adjustment screw for reflector, 10 Fixing screws for

lamp mount, 11 Socket for contact plug

Fig. 34 12 V 100 W lamp holder with halogen lamp

Download from Www.Somanuals.com. All Manuals Search And Download.

Assembling and exchanging incident light

lamps

Lamphousing 106 z L

Besides the halogen lamp, the following gas

discharge lamps can be used, which each

require different lamp holders (Fig. 35) and

power units:

Assembling and exchanging Hg and Xe lamps

Power units:

Hg and Xe lamps are powered by separate

power units.

Please make sure to read the special manuals

for these power units.

Type

Average life span

Hg ultra high pressure lamp 150 W (A.C.)

Xe high pressure lamp

Hg ultra high pressure lamp 100 W (D.C. stabilised/non-stabilised)

Hg ultra high pressure lamp 100 W (D.C. stabilised/non-stabilised,

type 103 W/2)

100 h

400 h

200 h

175 W (D.C. stabilised)

300 h

Fig. 35 Lamp holders for gas discharge lamps

1 Upper clamp, 2 Seal point of the burner, 3 Lower clamp, 4, 6 Lamp holder screws, 5 Socket for cut-out plug, 7 Protective cover

Hg 50

Xe 75

Hg 100

Stab.

Download from Www.Somanuals.com. All Manuals Search And Download.

Always insert the burner so that

n. b.:

It is extremely important to heed the follow-

ing advice!

1. the lettering is upright after insertion (dif-

ferent diameters of the metal base for the

Hg 100 and Xe 75 burners ensure that these

are always inserted the right way up).

Always disconnect the power unit from the

mains before assembling the lamphousing

106 z.

Wait for the lamphousing to cool down for at

least 15 minutes as otherwise it may explode.

Never touch glass parts of the burner with

your bare hands as finger perspiration burns

in.

2. if the bulb has a seal point (Fig. 35), the

burner is turned so that this point will be at

the side, not in the light path.

Wipe off any finger perspiration and dirt with

a clean cloth.

Adjust the lamps immediately after ignition.

Never look directly into the light path (risk of

glare).

Always wear the supplied gloves and face

mask when assembling Xe burners (risk of

explosion).

Avoid switching on and off frequently, as this

greatly reduces the life of the lamp.

Hot Hg lamps do not ignite again until they

have cooled down.

It is best to keep a record of the number of

hours a lamp has been in use (hour counter in

the power unit) and compare it with the

manufacturer’s specifications.

Spent burners become discoloured and

should be exchanged before the specified life

expectancy has expired.

Put the upper pin of the burner between the

clamps of the flexible power supply and clamp

with screw (33.5).

Unscrew the stud (35.3) in the holder slightly,

insert the lower end of the metal base and

retighten the stud.

To exchange the collector on the lamphousing

106 z:

Using the focusing knob (36.1), move the

collector to the rearmost position. Pull the

focusing knob of the collector outwards so that

the collector can be removed.

n. b.:

Make sure that the lamp base and the power

unit have the same number. If the lamp base is

marked L1, for example, L1 must also be set on

the power unit to make full use of the lamp and

not to shorten its life.

The LH 106 z L is opened by undoing the fixing

screws on the lid (11.10).

Pull the cut-out plug slightly out of its socket and

flip up lid (11.11, 11.1).

Download from Www.Somanuals.com. All Manuals Search And Download.

Move the collector to the front position with the Assembly of the tubes and tube adapter IR/R

focusing knob (36.1).

Using a screwdriver, slacken the clamp screw

(37.1; 38.1; 39.1) on the side of the tube change

mount on the stand, mount the tube or tube

adapter IR/R (clamp screw points to the right)

n. b.:

and align with edges parallel to the microscope

(the Siedentopf binocular points upwards in a

V shape). The guide pin in the tube mount of the

Remove the protective cover from the burner.

Put the lamp holder with burner inserted into the stand must fit into the groove of the tube change

lamphousing and secure with the screws interface or interface of the tube adapter IR/R.

(10.10). Try moving the collector (36.1): it must Retighten the clamp screw. The procedure is the

not touch the power lead. When closing the same for mounting the tube on the IR/R adapter.

lamphousing, make sure that the pins of the cut- Similarly, the DM R tubes can be connected via

out plug engage in the sockets (35.11). Retighten this adapter, e. g. the binocular observation and

the screws of the lid. Push the cut-out plug in as photo tube HC FSA 25 PE (41.1), viewing angle

far as it will go.

30°.

Attach the lamphousing to the microscope and With side port for reflecting measurement

connect to the power unit (compare mains scales and µ marks into the microscope image

voltage!).

(slide overlay) and for connecting the

MACRODUAL ZOOM device.

Field of view index up to 22.

Adjust the burner immediately after ignition.

Eyepiece diameter 30 mm for HL PLAN 10x/20 or

22 eyepieces.

Eyepieces with larger field of view numbers are

not recommended for use on the DM IRB.

The tube adapter IR HC is mounted on the tube

change mount of the stand and stabilised by

tightening the clamp screw.

Fig. 36 Lamphousing 106 z L with Hg 100 W lamp

1 Collector focusing, 2 Lamp adjustment, vertical, 3 Lamp

adjustment, horizontal,

adjustment (not visible)

Lamp holder Hg,

Reflector

Download from Www.Somanuals.com. All Manuals Search And Download.

Photo port for 1 photo/TV connection (43.2)

Photo port for 2 photo/TV connections (43.1)

Switchable 100 %/100 % (25.3)

n. b.:

Hold on to the tube adapter until the clamp

screw is tightened.

All Leica DM R trinocular tubes have the

following beamsplitting system:

100 % vis., 100 % photo or 50 %/50 %.

Then insert the HC FSA 25 PE tube in the change

mount of the tube adapter and fasten with clamp

screw.

The following tubes from the Leica DM R range

are also adaptable:

Bino HC BSA 25 (42.1)

Trino HC FSA 25 P and PR (42.2)

(P + PR = with and without back reflection)

Fig. 37 HCI B22, binocular tube with 45° viewing angle, field

of view up to 22 mm, eyepiece diameter 30 mm for HC PLAN

10x/20 or 22 eyepieces, interpupillary distance setting:

55 – 75 mm

1 Clamp screw, 2 Eyepiece port, 3 Siedentopf binocular part

Fig. 38 HCI 3T 22 trinocular tube with 45° viewing angle

Light path: 100 % vis

– switch rod

150 % – 50 % – switch rod

100 % – photo – switch rod

Field of view index up to 22, eyepiece diameter 30 mm for HC

PLAN 10x/20 or 22 eyepieces, interpupillary distance setting:

55 – 75 mm

1 Clamp screw, 2 Eyepiece port, 3 Siedentopf binocular part,

4 Photo/TV exit, 5 Switch rod

Fig. 39 HCI BV22, ergo binocular tube with 15°– 50° viewing

angle, field of view index up to 22, eyepiece diameter 30 mm

for HC PLAN 10x/20 or 22 eyepieces, interpupillary distance

setting: 55 – 75 mm

1 Clamp screw, 2 Eyepiece port, 3 Siedentopf binocular part

Download from Www.Somanuals.com. All Manuals Search And Download.

Fig. 41 Tubes from the DM R range

HC FSA 25 PE, Side port for optical overlay,

3 Tube adapter IR HC, 4 Clamp screw for mounting the

adapter to the stand, 5 Clamp screw for mounting the tube to

the adapter, 6 Photo/TV port

Fig. 40

1 Tube adapter R/IR HC, 2 Clamp screw

Fig. 42 Leica DM R HC tubes

1 HC BSA 25, 2 HC FSA 25 P + PR, 3 Beamsplitter switch rod,

4 Mount for photo adapter tube, 5 Clamp for photo adapter

tube, 6 Clickstop position for Pol eyepieces, 7 Socket for

control cable (PR tube only)

Fig. 43 Photo adapter tube

1 Photo adapter tube with 2 exits, 2 Photo adapter tube with

1 exit, 3 100 % ↑ / 100 % switch rod, 4 Clamp screw

Download from Www.Somanuals.com. All Manuals Search And Download.

Adaption of the slide overlay device and the

macro dual system

With the Leica DM IRB inverted microscope, the

slide overlay and macro devices can only be

adapted onto the FSA 25 PE tube.

This tube has a side flange (44.1) for mounting

the reflection optics. These reflection optics are

used for mechanical and optical adaption of the

slide overlay device and the macro dual zoom

system.

Fig. 44 Slide overlay device on the FSA 25 PE tube (with tube

adapter/45)

1 Tube flange, 2 Coupling ring of reflection optics, 3 Reflection

optics, 4 Coupling ring of slide overlay device, 5 Knurled

focusing ring, 6 Slide holder 5 x 5 cm, 7 Filter slot, 8 Illumina-

tion adapter tube of lamphousing

The slide overlay device consists of the

reflection optics (44.2), the illumination unit with

6 V 4 W halogen lamp (44.8), the standard

5 x 5 cm slide holder (44.6) and the control (44.5)

for focusing the slides. The halogen lamp is

Mount the reflection optics (44.3) onto the tube

flange (44.1) with the coupling ring (44.2),

ensuring that the guide pin engages in the

groove, and screw down. In the same way,

screw the slide overlay device with coupling

ring to the reflection optics, again watching the

position of the guide pin.

Fig. 46 Macro device on FSA 25 PE tube with tube adapter/45

1 Tube flange, 2 Coupling ring, 3 Reflection optics, 4 Coupling

ring, 5 Macro adapter, 6 Screw ring, 7 Zoom setting ring 1 : 4,

8 Scale of zoom factor, 9 Scale of magnification factor of the

working distance, 10 Scale of object distance from the bottom

edge of the mirror housing, 11 Mirror housing

Fig. 45 Transformer

7 9

1 2 3

10 11

Download from Www.Somanuals.com. All Manuals Search And Download.

Screw the reflection optics (46.3) to the tube

flange with the coupling ring (46.2). Align the

macro adapter (46.5) to the macro dual zoom

and secure with the screw ring (46.6). Screw the

macro adapter and macro dual zoom to the

reflection optics with the coupling ring.

Important!

Be very careful to keep the optical surfaces

clean. Any dust particles and finger marks will

show up in the image.

Check that the guide pin engages in the groove.

The graticule diameter for all HC PLAN eye-

pieces is 26 mm.

Inserting the eyepieces

Eyepieces 10x/20 M and 12.5/16 M only:

● Screw the retaining sleeve out of the under-

neath of the eyepiece.

The eyepieces are inserted into the eyepiece

tubes.

● Insert the graticule with the coated side

facing downwards (towards the objective). If

there is any lettering on the graticule it must

be imaged the right way round when viewed

in the later observation direction.

Use the following eyepieces only:

HC PLAN 10x/20

HC PLAN 10x/20 M

HC PLAN 10x/22

HC PLAN 10x/22 M

HC PLAN 12.5x/16 M

Widefield 16x/14 B (M)

● Screw the retaining sleeve back in.

Eyepieces 10x/22 M only:

● Screw out the underneath of the eyepiece.

● Remove the retainer ring inside it with a blunt

blade.

Widefield 25x/9.5 B (M)

(a spacer ring is required for the widefield objectives)

● Insert the graticule with the coated side

facing downwards (towards the objective). If

there is any lettering on the graticule it must

be imaged the right way round when viewed

in the later observation direction.

Information on the diameter, the visible area of

the specimen and the total magnification of the

microscope can be found in the chapter

“Technical data; performance data”.

● Screw the retainer ring back in.

Inserting graticules*

You can retrofit graticules yourself to the

HC PLAN eyepieces in the above list.

Graticules can only be inserted in eyepieces

with an adjustable eyelens = M type. The sec-

ond eyepiece should be an M type as well.

Download from Www.Somanuals.com. All Manuals Search And Download.

Inserting the photoeyepieces*

If any positions remain unoccupied, close them

with a screw cover to prevent dust penetrating

the microscope optics.

The HC PLAN observation eyepieces (slot-in

diameter 30 mm) are designed for direct visual

observation. For the adaption of photo-micro-

graphic equipment with a fixed magnification

factor, e. g. DM LD and MPS systems, and for

special TV adaption systems, special eyepieces

with a slot-in diameter of 27 mm and the

engraving HC...PHOTO are used (note the

adapter!)

Please note that the front lenses of the

objectives point upwards and are therefore

more exposed to contamination than those on

upright microscopes.

Therefore check fairly frequently that the front

lens is clean.

See special manual for further details on

adapting photo and video equipment.

A constantly updated optics sheet outlines the

current range of objectives that can be used on

the DM IRB/E. Ask your Leica agency for a copy.

Screwing objectives in and out

Assembling the stages, the plane stage and

object guide

For the electronic version of the microscope, the

DM IRB/E, the objectives are screwed in during

the initial installation (see relevant chapter). For

the manual version, proceed as follows:

Plane stage

The plane stage is fixed to the microscope with

3 screws (48.4). The object guide can be

Remove the screw covers from the objective mounted to either the right or the left of the

threads.

plane stage (48.2).

Screw the objectives into the openings in the

eyepiece so that the magnification can be

changed in steps (e.g. in the order 4, 10, 20, 40).

Fig. 48 Plane stage

1 Insert ring, 20/40 mm diameter, 2 Drill holes for mountable

object guide, 3 Drill holes for specimen clips, 4 Drill holes for

securing the stage

Fig. 47

Download from Www.Somanuals.com. All Manuals Search And Download.

3-plate x/y stage

To assemble the square insert plate:

The 3-plate x/y stage no. 19, size 247 x 230 mm,

x-y adjustment range 60 x 40 mm, is delivered in

separate packaging and assembled as follows:

1. Insert the corner of the insert plate that is

marked red (50.5) at an angle from above into

the corner of the stage that is also marked

red and is fitted with a spring (50.5).

This stage is usually delivered with the DM IRM,

so the description of its assembly has been

taken from the DM IRM manual!

n. b.:

Only press the spring at the side!

1. Screw the 3 Allen screws out of the stage

support surfaces and wipe any remains of

packaging or dust, etc. from the stage with a

clean cloth.

2. Align the stage with the x-y drive (49.1) at the

front right and lay it so that its undersurface

rests on the stage support surfaces.

3. Align the drill holes in the stage over those in

the support surface. If the drill holes are

covered, please adjust the upper stage plate

with the x-y stage drive.

Do not press the square insert plate onto the

spring from above, as the insert will then not

be aligned plane-parallel to the stage and

can be bent.

2. Drill holes (50.2) for attaching small biological

specimen clips.

3. Insert the round stage inserts into the

opening (50.1).

4. Screw down the stage with Allen screws.

Fig. 49 3-plate x/y stage no. 19 without inserts

1 Stage drive, 2 Rear fixing holes, 3 Front fixing hole (not

visible, concealed by stage plate), 4 Corner with red dot and

spring

Fig. 50 3-plate x/y stage

1 Insert ring, 20/40 mm diameter, 2 Drill holes for specimen

clips, 3 Drill holes for securing the stage, 4 Coaxial drive for

specimen positioning with universal joint, 5 Red markings

Download from Www.Somanuals.com. All Manuals Search And Download.

Rotary stage and insert frame for coverslips

Connecting the microscope to the mains

The rotary stage is secured with 3 screws. The When you have installed all the components as

rotary mount has to be moved to make all the described, you can connect the microscope to

screw holes accessible. Align the screws (51.2). the power supply with the mains cable.

If you have the manual version of the DM IRB,

installation is now complete and you can jump to

the “Operation” chapter.

n. b.:

Washers (51.3) should be used as well for the

back drill holes. Only screw the screws in lightly,

as the rotary stage first has to be pressed into

the centre. This is done by inserting the

centration aid (51.4) into the rotary stage.

Engage the Bertrand lens by turning the knurl

and focus with the lever. Move the stage until

the bright circle is in the centre of the field

of view. Then secure the stage in position,

disengage the Bertrand lens and remove the

centration aid. To secure specimen slides in the

frame inserts (52.1), press on the middle of the

leaf spring (52.2) and slide in the coverslip in the

direction of the arrow. Clamp the frame insert in

the object guide (51.1).

If you have a DM IRB/E model (i. e. the electronic

version), you have to set up the system.

The following chapter describes how to set

up the electronic version of the DM IR: the

DM IRB/E.

Fig. 51 Rotary stage

1 Object guide, 2 Screws for securing the stage, 3 Washers,

4 Centration aid

Fig. 52

1 Frame insert for coverslips, 2 Leaf spring

ᮢ

Download from Www.Somanuals.com. All Manuals Search And Download.

The E version DM IRB/E

For users who want to program the Leica

DM IRB/E microscope themselves, free

software development kit called “Leica SDK“ is

available for Windows 3.11, Windows 95 and

Windows NT on request.

Features of the Leica DM IRB/E

The Leica DM IRB/E offers the following addi-

tional functions:

– Motorised, sextuple objective nosepiece

– Electronic focusing

Important note:

Before using a brand new Leica DM IRB/E

microscope for the first time, an initial

installation has to be carried out.

– Coding of the IC objective prisms*

– Motorised fluorescence filter cube change

with electrically operated dark flap*

– Control panel for remote control of the

microscope components*

– Footswitch* (in connection with control panel

or DM STC stage drive)

– LC display of microscope functions.

Function and operation

Straight after the Leica DM IRB/E microscope is

switched on, the system will be initialised. This

takes a few seconds. A message to this effect

will appear in the LC display on the front of the

microscope.

This manual applies to the Leica DM IRB/E

inverted research microscope with Eprom

version numbers:

Master (M)

Nosepiece (R) 2.30

Z drive (Z) 2.40

2.40

Deviations will therefore naturally occur for

previous or subsequent versions.

* The EPROM version number is displayed by simultaneously

pressing the “LEARN“ and “CHANGE“ keys (Fig. 53b), and

by pressing the “CHANGE” key afterwards the 3 EPROMS

can be read out in succession.

Download from Www.Somanuals.com. All Manuals Search And Download.

Assembly and initial installation

If necessary, delete them by sustaining the rele-

vant keys ( , Fig. 53b) for longer than

1 sec. Using the lower focus key, behind the

handwheel on the right, move the nosepiece to

the lower stop.

The assembly of the individual components,

such as transmitted light illumination column,

condenser, etc. has been completed.

n. b.:

The objectives should not be screwed in at this

point. The best time to do this is when executing

the learn mode.

Generally you are free to choose the order in

which the separate steps of the learn mode are

carried out. However, for the first installation we

recommend you keep to the following order:

Check that the focus threshold

and the lower threshold

are deleted, i. e. neither of the two symbols may

appear in the display.

Fig. 53b Controls

Fig. 53a

- 1 . 86mm S1

100xPH3 HH

LEARN CHANGE

STEP

see

Fig. 53b

Lower Z position

Focus position

Focus stepwidth

(S0, S1, S2, S3, SC)

Download from Www.Somanuals.com. All Manuals Search And Download.

Learn mode

After switching on, the microscope is in the nor- The “EXIT” option in the display flashes.

mal operation mode.

If you press the “LEARN” key again, the current

objective is rotated back into the observation

position without any parameters being changed.

Besides this, you automatically switch back to

the normal operation mode.

µm

100xPH3

Installing the objective prisms

If your system is not equipped for interference

contrast, skip this section and the next and

continue reading at “Installing the objectives”.

The IC objective prisms are normally put in the

turret at the factory. If you are retrofitting TL

interference contrast, refer to the instructions

on page 16 of the DM IRB manual.

Normal operation mode

The learn mode is switched on with the

“LEARN” key.

The objective nosepiece rotates through 180° so

that the current objective is in the most

accessible position (furthest to the right on the

outside).

Learning the IC objective prisms (IC turret)

This function also serves for cleaning, assem-

bling, immersing, etc. the objective.

Using the “CHANGE” key, select the ICT option

in the Learn mode. Keep pressing the

“CHANGE” key until the second learn menu

appears and the ICT position in the display

flashes.

Lea r n:

PARF OBJ L /R

E X I T

Confirm by pressing the “LEARN” key.

Lea r n:

E X I T

I CT

FLUO

Input menu of learn mode

Download from Www.Somanuals.com. All Manuals Search And Download.

Turn the IC turret (situated under the objective Installing the objectives

nosepiece) until it clicks into the brightfield

position (H).

Select the “Objectives” option in the Learn

mode (OBJ) by pressing the “CHANGE” key; the

“OBJ” option now flashes.

Operate the focus handwheel until the letter H

appears on the display panel as well. Turn the IC

turret by a quarter of a rotation into the next

clickstop area. The message “IC prism 2”

appears in the display. Read the marking on the

turret and set the electronic display to the same

code by turning the handwheel. Do this for all

four positions. Empty positions should be coded

“–”.

Confirm your choice with the “LEARN” key.

Ob j e c t i v e 1 :

5x PH

Learn mode: Objective data – Magnification

Select objective “1” by pressing the objective

change keys (behind the focus handwheel on

the left).

I C Pr i sm 1 :

Learn mode: IC turret

Conclude the Learn mode for the IC turret by

pressing the “CHANGE” key. “EXIT” flashes in

the display; confirm with the “LEARN” key.

Download from Www.Somanuals.com. All Manuals Search And Download.

Now screw the objective with the lowest Display: IC objective prism

magnification into the nosepiece opening which By pressing the “CHANGE” key, select the

is furthest to the right.

display field for the IC objective prism.

Display: Objective magnification

By turning the focus handwheel, select the

number in the electronic display that corre-

sponds to the magnification of the objective.

Ob j e c t i v e 1 :

5xPH

Display: Phase contrast

By pressing the “CHANGE” key, select the dis-

play field for phase contrast.

Learn mode: Objective data – IC coding (code letter)

By turning the focus handwheel, select the

display that corresponds to the top line of

engraving on the objective (A, B, C, D, E, F). The

Ob j e c t i v e 1 :

symbol “H” (Hellfeld,

brightfield), is for

objectives that are not suitable for IC.

The choice of objective prism that can be set

here is confined to the IC prisms that are

actually on the IC turret and that have been

learned.

5x PHO

Learn mode: Objective data – Phase contrast

By turning the focus handwheel, select the

display that corresponds to the engraving on the

objective (PH1, PH2 . . .). The symbol “--” is for

brightfield objectives.

Download from Www.Somanuals.com. All Manuals Search And Download.

To learn further objectives:

Operating modes: Dry/Immersion

Select objective no. 2 with the upper objective

nosepiece key. Screw the objective with the

next highest magnification into the nosepiece

opening which is now furthest to the right.

To ensure simple yet safe objective change, the

objectives have to be classified in one of the

following three categories:

1. Dry objectives (D) = all dry objectives with a

short working distance (< = 3 mm).

2. Immersion objectives (I).

3. Combined objectives (C) = dry objectives with

a long free working distance (> 3 mm), ob-

jectives which can be used for scanning

purposes as well through an oil layer.

By turning the handwheel, select the magnifi-

cation display that matches the objective, as

you did for the first objective. Proceed in the

same way for setting the Phaco display, the IC

prism display and the operating mode. Then

repeat the setting procedures for the other

objectives.

Nosepiece positions that are not occupied by an

objective are given the code “--”. This has the

effect that these positions are not travelled to in

standard mode.

By pressing the “CHANGE” key, select the

display box for the operating mode.

Now select the valid objective category for the

objective you are using (D, I, C) by turning the

focus handwheel.

Conclude the Learn mode for the objective

parameters by pressing the “CHANGE” key.

“EXIT” flashes in the display; confirm with the

“LEARN” key.

Ob j e c t i v e 1 :

5xPH

Before selecting the “Parfocality” option in the

Learn mode, you should take the following

steps:

Learn mode: Objective data – Operating mode

– If you want to use a specimen holder on your

stage, fit it now.

– Put a specimen on the stage.

– Switch to the highest magnification and focus

the image of the specimen.

Now all the objective parameters for the first

objective have been learned, and the other

objectives can be installed.

– Set the focus position with the

key

(→ Fig. 53b).

You can now begin with learning the parfocality.

Download from Www.Somanuals.com. All Manuals Search And Download.

Parfocality

Oil immersion objectives

Select the “Parfocality” option (“PARF”) in the Once the parfocality has been learnt for all the

Learn mode by pressing the “CHANGE” key; the dry objectives it can be done for the immersion

“PARF” option now flashes.

objectives.

Confirm your choice with the “LEARN” key.

Please remember that if the specimen is very

small and lightweight it must be fixed onto the

stage to prevent it being moved by the adhesive

force of the oil.

Select the oil immersion objective with the

objective changing key.

Apply a drop of immersion oil to this objective.

To do this, you can move the objective to the

most easily accessed outer position with one of

the objective changing keys.

Return the objective to the working position and

focus.

Confirm the learnt focus position by pressing the

“LEARN” key. “ADJUSTED” appears in the dis-

play.

Proceed similarly for any other immersion ob-

jectives.

1 : 5x S1 E

ADJUST & LEARN

Learn mode: Parfocality

Dry objectives

Select the dry objective with the highest magni-

fication by pressing the objective nosepiece

keys (behind the focus handwheel on the left).

Focus the specimen with the focus handwheel.

Use the “STEP” key to select the suitable focus

stepwidth, also using the focus keys if neces-

sary (behind the handwheel on the right). When

the focus position is set, the z drive stops near

the focal plane.

Conclude the parfocality setting by selecting the

“EXIT” option with the “CHANGE” key and

confirming with “LEARN”.

Confirm the learned focus position by pressing

the “LEARN” key. “ADJUSTED” appears in the

display.

Exiting the Learn mode

Now select the dry objective with the next lower

magnification.

To leave the Learn mode, select “EXIT” and

confirm with “LEARN”.

Focus the specimen again with the focus hand-

wheel and confirm with “LEARN”.

Repeat this procedure until you have reached

the smallest dry objective.

For low-power dry objectives (5x, 10x) it is ad-

visable not to correct the focus any further, as

these objectives are focused immediately after

switching on.

Download from Www.Somanuals.com. All Manuals Search And Download.

Individual user adjustments

Z-drive Objective Nosepiece move-

keys

ment when the

right handwheel is

turned clockwise

Chosen function

Select the option “L/R” in the Learn mode by

pressing the “CHANGE” key and confirm your

choice by pressing the “LEARN” key.

Ob j e c t . < > F o c u s

right

left

down

F o c u s < > Ob j e c t .

left

right

i t c he s :

down

Possible combinations for user adjustment

b j ec t .

Focus

By turning the focus wheel, choose the one of

the four possible combinations that suits you

best.

Learn mode: User adjustment

This option allows you to choose whether you

want to operate the objective nosepiece on the

left or the right side of the microscope. The

function of the focus keys then also shifts to the

other side of the microscope.

The standard setting made at the factory is as

follows (= combination 1 in the above table):

– The nosepiece is operated on the left side of

the microscope; accordingly the keys for

lowering and refocusing are on the right.

It is also possible to reverse the rotation direc-

tion of the handwheel and its effect on the

focusing direction.

– Rotation direction of the handwheel and fo-

cusing movement:

If the handwheel on the right of the micro-

scope is rotated clockwise, the objective

nosepiece is moved upwards, i. e. the objec-

tive moves towards the sample.

Conclude the Learn mode for user adjustment by

pressing the “CHANGE” key. “EXIT” flashes in

the display; confirm with “LEARN”.

Download from Www.Somanuals.com. All Manuals Search And Download.

Installing the fluorescence filter cube

Learning other filters:

Select the next filter cube position by pressing

one of the “FLUO” keys on the control panel of

the FLUO module. Insert the filter cube and

select the corresponding name in the display.

Repeat the procedure for any other filters.

Unoccupied positions are given the code “-”.

Select the “FLUO” option in the Learn mode by

pressing the “CHANGE” key. Confirm by press-

ing the “LEARN” key.

Fi l terbl ock 1:

To conclude the Learn mode for the fluores-

cence module, press the “CHANGE” key. “EXIT”

then flashes in the display; confirm with

“LEARN”.

Now leave the learn mode by pressing “EXIT”

and confirming with “LEARN”.

EX I T

I 3

Learn mode: Fluorescence

Pull out the filter cube drawer on the left side of

the microscope stand and put the filter cube you

want to use in the holder of the fluorescence

turret plate in the light path. The filter cube must

click noticeably in position.

Now select the corresponding filter cube name

in the LC display by rotating the focus hand-

wheel.

Concluding the installation

Installation is now complete. You are back in the

normal operation mode.

Before you start work, you should set the focus

threshold with one of your specimen slides.

(with the

key, → Fig. 53b).

Download from Www.Somanuals.com. All Manuals Search And Download.

Motorized objective nosepiece

The upper key is pressed to increase the

magnification, the lower key to decrease the

magnification. Short pressure on the key

switches to the next lower or higher

magnification. If you sustain the key for longer

then 0.3 sec., the display jumps to the next

higher or lower magnification every 0.5 sec. The

nosepiece is not actually turned until you

choose a specific magnification by releasing the

key. All you have to do to switch from any higher

magnification down to survey magnification, for

example, is to sustain the lower objective key for

approx. 3 sec. The selected objective is turned

into the light path in the direction that involves

the shortest travel distance.

The electronic nosepiece control allows easy

and safe change of the objective magnification.

Objectives are changed with 2 push buttons

(objective changing keys) which are easily

accessed behind the focusing handwheel on the

left of the microscope.

n. b.:

When installing the system (see “Individual user

adjustments”, → p. 48) it is possible to operate

the objective change function on the right side

of the microscope instead of the left.

Left side of microscope

Download from Www.Somanuals.com. All Manuals Search And Download.

Operating modes

DRY and IMMERSION

Contamination of the dry objectives is prevented

by the fact that the objective nosepiece is

always lowered before objectives are changed.

The Leica DM IRB microscope is equipped with

a switch function between the operating modes

“Dry” and “Immmersion” (IMM) to ensure

straightforward, error-free operation.

This prevents

When switching from one operating mode to the

other, please proceed as follows:

Starting in the DRY mode:

(Display at the bottom right in the LD display: D)

Press the keys “lower z position”

”focus position” on the control panel of the

microscope simultaneously to switch from Dry

to Immersion.

– dry objectives from being immersed in oil by

mistake

– immersion objectives from being used without

immersion oil by mistake.

and

Changing the operating mode

The operating modes are switched by simulta-

neously pressing the keys “lower z position”

The objective nosepiece is lowered and the

message “Change Objective” appears in the

display. The corresponding oil immersion

objective is switched into the light path with the

objective changing key (normally the upper

objective changing key). From now on, only oil

immersion objectives or objectives of the

“Combined” category are travelled to.

and “focus position”

on the control

panel of the microscope. This means that

immersion cannot be travelled to in Dry mode

and dry objectives with a short working distance

cannot be travelled to in Immersion mode.

To ensure smooth operation, immersion oil must

be applied to all oil immersion objectives in the

nosepiece that are to be used before you start

work.

(“I” now appears in the LC display at the bottom

right).

Download from Www.Somanuals.com. All Manuals Search And Download.

The procedure for switching to the Dry mode is Automatic lowering of the objective nosepiece

analogous:

Again, press the keys “lower z position”

and “focus position”

of the microscope simultaneously to switch from

“Immersion” to “Dry”.

In order to be able to operate the objective

changing keys easily and without touching the

on the control panel

stage in situations where space is difficult, e.g. if

there are small object inserts in the stage and/or

if the specimen plane is relatively high above the

stage level, the objective nosepiece is lowered

before it is rotated. The end position for this

lowering (= lower z position ↓_) can be chosen by

the user.

If the lower z position is not set, the objective

nosepiece is lowered by the maximum possible

distance.

The objective nosepiece is lowered and the

message “Change Objective” appears in the

display. You now have the opportunity to put a

new specimen slide (without immersion oil) on

the stage. Then, using the objective changing

key, switch the appropriate dry objective into

the light path (normally the lower objective

changing key). From now on, only dry objectives

or objectives of the “Combined” category are

travelled to.

(“D” now appears in the LC display at the bottom

right).

To learn objective categories, see “Installing the

objectives” (→ p. 44).

Download from Www.Somanuals.com. All Manuals Search And Download.

Brightness adjustment

Instead of a potentiometer, Leica DM IRB/E The lamp intensity is adjusted by rotating an

microscopes are equipped with an incremental adjustment wheel on the left side of the

transducer for brightness adjustment. This microscope. If you hadn’t already switched to

means that the adjustment wheel is automati- the lamp voltage display, this will happen

cally moved from clickstop to clickstop and automatically when you move the wheel.

therefore has no end stops.

Similarly, the display will switch to the Z position

if the Z position is changed with the handwheel

After it is switched on, the microscope is in nor- or by pressing the key.

mal operating mode. In the first line of the LC

display, the current Z position is given in µm or To switch off the transmitted light illumination,

mm on the left.

the brightness is first reduced to 2.5 V. Then

You can switch to the lamp voltage display by rotate beyond the lower value.

pressing the “CHANGE” key on the control To switch on again, rotate briefly in the opposite

panel. The lamp voltage is displayed in volt from direction.

2.5 – 12 V. If you press the “CHANGE” key again

you return to the display of the Z position.

For photomicrography we recommend a setting

of 10.5 V.

Download from Www.Somanuals.com. All Manuals Search And Download.

Electronic focus

The electronic focus offers the user the follow- The controls of the electronic focus are:

ing advantages:

– The focusing handwheels, conventionally

– Extremely sensitive focusing, especially for

high magnifications.

positioned on both sides of the microscope.

– Two keys (focus keys) for fast lowering of the

objective nosepiece and returning to the focal

plane. The keys are in a convenient position in

front of the right handwheel.

– Fine focusing selectable in 4 steps; coarse

focusing can be switched on “blind” at any

time.

The stepwidths desired by the user (corre-

sponding to gear ratio and sensitivity) are

allocated to every single objective and

automatically reset as soon as the particular

objective is used.

If both focus keys are pressed simultaneously,

coarse focusing is switched (SC). The coarse

focus is switched off again the moment that

the two keys are pressed simultaneously

again, or a different focusing speed is switched

with the STEP key.

– Fast lowering of the objective nosepiece and

exact repositioning to the previously set focal – Key for switching the focusing speed (“STEP”,

plane.

S0 = 0.05 µm, S1 = 0.1 µm, S2 = 0.7 µm,

S3 = 1.5 µm) on the front of the microscope.

The micrometer values always indicate the

smallest stepwidth that can be carried out.

– Electronic parfocality of all objectives through

intelligent linking of motorized objective nose-

piece and electronic focus drive.

Download from Www.Somanuals.com. All Manuals Search And Download.

– Key

for defining the “lower Z position”.

Coded IC objective prisms (option)

Pressing the key for longer than 1 sec. deletes

the threshold; another press of the key for

longer than 1 sec. sets the current Z position

as “lower threshold”.

The objective IC prisms are arranged on a turret

underneath the objective nosepiece. To facili-

tate allocation and thus the setting of the

objective prisms to the objectives, the LC display

on the front of the microscope indicates both

the IC prism required for the objective in the

light path and the currently effective IC prism on

the turret.

– Key

for defining the “upper Z threshold”

(= focus position). Pressing the key for longer

than 1 sec. deletes the threshold, another

press of the key for longer than 1 sec. sets the

current Z position as “upper Z threshold”.

The latter flashes if the combination is wrong.

n. b.:

If using the microscope without the stage plate,

please note that when you replace the front

fixing screw if the stage plate, it must not be

screwed in fully. If it is screwed in too far it will

block the focus motor. The message BLK then

appears in the LC display on the front of the

microscope.

Download from Www.Somanuals.com. All Manuals Search And Download.

Motorised fluorescence filter cube change

(option)

Three keys are used for operation:

A control panel is connected to the Leica

DM IRB for motorised filter cube change.

Mot. filter cube module

Shutter filter module

RS 232 control F-bus

panel

F-bus F-bus

in out

Mains

unit

Back of

Leica DM IRB

Control panel

for mot. filter cube change

Connection for motorised fluorescence filter cube change

The two “FLUOR” keys are used to switch to the

adjacent filter cube. If you switch one of these

keys twice, you switch by two filter cube po-

sitions.

The “SHUTTER CLOSED” key is used for opening

and closing the electric shutter. The LED

indicates whether the shutter is closed or not

(LED lights up when closed).

Download from Www.Somanuals.com. All Manuals Search And Download.

Control unit*

When using a Leica DM IRB microscope, the

unit must be powered by an external power unit.

The switch on the back of the unit must

therefore be at EXT, and voltage is supplied via

an ordinary 7 V plug-in-power supply.

An electronic control unit can be connected for

the remote control of individual microscope

components such as objective nosepiece and Z

drive.

(Mains unit: 7 – 15 V, pole direction

I = > 100 mA).

It is operated with keys on the front of the

control unit.

The supplied connecting cable is connected to

the MICROSCOPE socket on the control panel

and CONTROLPANEL on the microsope.

The CONTROLPANEL interface on the micro-

scope is active from Eprom version number 2.4

upwards.

The control unit can also be combined with a

dual footswitch.

Connecting the control unit

The connection port is on the back of the unit.

Optionally, a dual footswitch, order no. 505 096,

can be connected to the FOOTSWITCH socket.

POWER

MICROSCOPE

15 V DC EXT INT

FOOTSWITCH

Connection port of the control unit

Download from Www.Somanuals.com. All Manuals Search And Download.

Keys on the control unit

The control unit is operated by keys.

FILTER

LAMP

FIELD

MAG

FLUOR

FOCUS

OFF

COND

LAST

STEP

SHUT

LAST

SPEED

FOOT

X – Y

Keys on the control unit

The keys can be used to operate the following microscope components:

Component

Name on the unit

Filter

FILTER ((in preparation)

Lamp

LAMP

COND

FIELD

Condenser

function not

possible on

Leica DM IRB/E

Field diaphragm

Aperture diaphragm

Objective change

Incident light fluroescence axis

Z drive

MAG

FLUOR

FOCUS

X-Y

x-y stage

Footswitch

FOOT

Several keys are allocated to each component. These are grouped together on the key panel.

Download from Www.Somanuals.com. All Manuals Search And Download.

The control unit is operated in a similar way to This is also the reason why the nosepiece

the key-mode operation of the Leica DM IRB rotation is slightly delayed after the keys are

microscope.

pressed.

The LAST key is used to switch to the objective

that was used last.

Significance of individual keys:

LAMP

FLUOR

Brightness can be adjusted with the arrow keys: The arrow keys are used to switch from one

Up arrow → brighter, down arrow → darker. filter cube to the next.

The ON/OFF key is used to switch the lamp off Pressing the keys several times causes the

and on. When it is switched on again, the corresponding position to be jumped to.

brightness that was set before it was switched The LAST key positions the filter cube that was

off is reset.

set before the last keystroke.

The SHUT key is used to close the dark flap.

MAG

The arrow keys are pressed to change the

magnification. The objective nosepiece rotates

to the next higher magnification within the same

operating mode (IMM/DRY) when the upwards

arrow key is pressed once. Similarly, the next

lower magnification is switched to when the

downwards arrow is pressed. If the same key is

pressed more than once in quick succession,

the nosepiece is rotated by the corresponding

number of valid positions.

Download from Www.Somanuals.com. All Manuals Search And Download.

FOCUS

Here the arrow keys control the movement of The arrow keys define the direction in which the

the Z drive. stage is to move.

X-Y

The movement speed for the fine focusing is Two different speed modes can be preselected

selected with the STEP key. For safety reasons, (Slowmode/Fastmode) with the SPEED key. The

the movement range is limited to 400 µm above travel speed is increased as a function of the

the focus position and 400 µm below the lower speed mode with a predefined ramp: the longer

Z position.

one of the arrow keys is pressed, the faster the

If both arrow keys are pressed at the same time, stage moves. The speed mode determines the

the system switches to coarse focusing (SC). maximum speed.

The Z drive then moves at a higher speed. Here,

the movement range is limited by the set FOOT

threshold (lower Z position) and at the top end

The left footswitch is assigned a function via

by the focal plane (focus position), to prevent

key 1, the right footswitch via key 2.

collision damage. The coarse focus is switched

After the microscope is switched on, the foot-

off the moment the two arrow keys are pressed

switches have the default function Magnifi-

simultaneously again or a specific focus speed

cation change.

is selected with the STEP key.

The AF key is not assigned yet.

To assign a different function, first press key 1 or

2 and then the desired function key. An acoustic

signal is given for correct inputs.

Download from Www.Somanuals.com. All Manuals Search And Download.

Examples for the use of the footswitches

Example 2

Example 1

Switching between two fluorescence filters with

a footswitch:

Switching between two magnifications with a

footswitch:

Set a filter cube with the arrow keys in the FLUO

box and switch to a second filter cube you

would like to use. Assign the LAST function for

filter cube change to the right footswitch, by

first pressing key 2 and then the LAST key (at

FLUO). Now switch between the two

fluorescence filters with the right footswitch.

Set a magnification and switch to a second

magnification you would like to use. Assign the

LAST function for magnification change to the

left, by first pressing key 1 and then the LAST

key (at MAG). Now switch between the two

magnifications with the left footswitch.

Download from Www.Somanuals.com. All Manuals Search And Download.

Person sensor (option)

Switch position AUTO

The dark flap can be opened automatically via a

sensor when you look through the microscope

and closed again when you look away.

The dark flap is opened when you look through

the eyepiece. When you move away, it will be

closed again after about 3 seconds.

This is achieved by mounting the person sensor

(order no. 505 101) onto the right eyepiece tube

and connecting the cable on the sensor to the

SHUTTER socket on the control unit for motor-

ised filter cube change.

Switch position LIGHT ON

The dark flap remains open (e. g. for photo-

micrography). If the flap is still closed, it must be

opened first manually. It will then remain open.

Switch position SENSOR OFF

The person sensor is switched off. The position

of the dark flap remains unchanged.

SHUTTER

CLOSED

FLUOR

Person sensor port

n. b.:

The diode next to SHUTTER CLOSED indicates

whether the dark flap is currently open (diode

off) or closed (diode on).

You can control the dark flap manually by

pressing the key above SHUTTER CLOSED or

with the SHUT key on the control unit.

The shutter key on the microscope or on the

control unit can be operated at any time. The

SHUTTER CLOSED diode indicates whether the

flap is currently open or closed.

If the dark flap is closed before the automatic

functions are switched on, it has to be opened

manually before the automatic functions take

effect.

When the microscope is switched on the dark

flap is closed at first. To activate the person

sensor you first have to open the dark flap

manually.

FLUO

LIGHT

AUTO

SENSOR

OFF

Front view of the person sensor

Download from Www.Somanuals.com. All Manuals Search And Download.

Front controls

There is an LC display and five control keys on the front of the microscope.

lower Z position

set

focus position

set

focus keys on the right

side of the microscope

Z position

stepwidth

- 1 . 86mm S1

100 xPH3 HH

fluo cube

current

Objective data

operating mode dry/imm

IC prism or

brightfield

LC display

The display gives information on the following At the factory the microscope is set so that the

functions:

fast focusing is controlled with the two keys on

the right side of the microscope, indicated by

the symbol on the right in the upper line of the

– Z position in µm or mm.

– Set stepwidth for the fine focusing (S0, S1, display.

S2, S3 and coarse focusing = SC, can be Similarly, if this symbol appears on the left side

switched on and off by simultaneously of the display, it means that the focusing is

pressing both focus keys).

controlled with the two keys on the left of the

– Lower

position set (symbol visible

microscope.

threshold set).

– Focus position set (symbol visible = threshold n. b.:

set).

When the microscope is installed the influence

– Objective data (corresponding to the objec- of the rotation direction of the handwheel on the

tive engraving).

focusing direction of the objective nosepiece

– magnification

can be reversed (→ p. 48).

– Phaco (PH1, PH2, . . . )

– required objective IC prism (option).

– Currently switched IC prism or brightfield

position.

– Fluo cube (option).

– Operating mode (dry or immersion).

Download from Www.Somanuals.com. All Manuals Search And Download.

Operation

Basic setting

for transmitted light

n. b.:

Switching on the halogen lamp

Switch on the 12 V 100 W lamp at the mains

switch (55.7).

When using acids or other aggressive

chemicals, special care should be taken.

Avoid direct contact of these substances

with optical and mechanical components.

Adjust the brightness with the dial. The numbers

are not absolute parameters, but merely serve

for reproducible setting. The white dot on the

dial indicates the setting for approx. 3200 °K for

photography on artificial light film and for TV

microscopy.

Fig. 54 – 55

1 Binocular phototube, 2 Eyepiece tube, 3 Eyepieces, 4 Tube mount (tube interface), 5 Tube port for photo/TV connection,

Beamsplitter switch, 7 Mains switch, 8 Brightness adjustment, 9 Lateral TV port, 10 Coaxial coarse and fine drive,

11 Fluorescence module, 12 ICT prism adjustment, 13 Sextuple objective nosepiece, 14 Centring buttons for incident light field

diaphragm, 15 Field diaphragm adjustment, 16 Filters, 17 Aperture diaphragm adjustment, 18 Lamphousing mount (or

mirrorhousing for two lamphousings), 19 Lamphousing, 20 Stage plate, 21 Analyser, 22 Tube lens module (Bertrand lens and

magnification changer), 23 Switch rod for lateral TV port, 24 Transmitted light illumination column, 25 Condenser, 26 Transmitted

light lamphousing, 27 Transmitted light field diaphragm, 28 SLR port, 29 Second lamphousing

Fig. 54 View from right side of microscope

Fig. 55 View from left side of microscope

Download from Www.Somanuals.com. All Manuals Search And Download.

Adjustment specimen

Caution!

For initial microscope adjustment we recom-

mend you use a specimen that has both high

and low contrast areas.

Please be careful with high objective magni-

fications when focusing or making x-y adjust-

ments!

It is easier to focus incident light fluorescence

specimens in transmitted light first.

When using objectives with a high magnification

and a short working distance (from 50x), the

specimen and the stage insert may be lifted and

tilted.

When scanning the specimen, the front lens of

the objective may knock against the edge of the

stage insert.

Focusing the specimen

(For the DM IRB version, please read the section about the

operation of the E focus and objective nosepiece first. Here,

an example of manual operation is given for each case.)

Lower the coarse and fine drive if possible when

turning the nosepiece and changing the ob-

jectives, in order to avoid contact between the

front lens and the stage insert.

Focus the specimen you want to examine. To do

this, the objective nosepiece should be lowered

first. The objective is turned into the light path by

rotating the black knurled knob on the nose-

piece. The objective should click audibly into

position.

n. b.

Caution with special objectives! Here there may

be contact between the stage insert and the

front lens the moment the objective is moved

over the edge of the inner hole of the stage

insert!

Focus the specimen with the coarse and fine

drive, which changes the height of the objec-

tive nosepiece. The stage height remains un-

changed. The total vertical travel of the

nosepiece is 7 mm. In air, the focusing range

extends from 2 mm below the stage surface to

5 mm above it.

One drum interval of the fine focusing cor-

responds to about 2 mm of the objective nose-

piece.

Download from Www.Somanuals.com. All Manuals Search And Download.

Checking of various microscope components

Engage or disengage the filters (54.16) accord- Roughly set the correction mount to the thick-

ing to the required brightness. ness of the base of the vessel on the stage by

If necessary, disengage the Bertrand lens by turning the knurled ring. Focus the specimen

turning the knurled knob (54.22), pos. 1. with the coarse and fine drive. Then operate the

Disengage the analyser (55.21), if necessary, by correction mount until you achieve the greatest

pulling it out partway. image contrast, using the fine focus if neces-

Operation of L objectives with correction mount

Disengage the filter systems, if necessary, by sary.

rotating the turret (55.11).

Push in the switch rod(s) for the beamsplitter Setting the tubes and eyepieces

(54.23).

Eyeglass wearers must remove (for 10x/25) or

Clamp the transmitted light illumination arm with

the knurled wheel (5.1).

push back (for 10x/20 and 10x/22) the anti-glare

protection of the eyepieces, but it should always

be left on for viewers not wearing eyeglasses.

● Set the interpupillary distance on the tube by

pulling apart or pushing together the eyepiece

tubes until only one image can be seen with

both eyes.

● Note your personal interpupillary distance.

● If using ergotubes, set the viewing angle

(15° – 50°) as well by tilting the binocular

viewing port. To avoid symptoms of fatigue,

vary the viewing angle from time to time.

● Close any tube exits you are not using to

prevent stray light disturbing the image.

Fig. 56 Examples of objectives

1, 2 Objectives with correction mounts (Corr) for adjusting

to different vessel base thicknesses (e. g. 0.1 – 1.3 mm and

0 – 2 mm),

Objective with built-in iris diaphragm (1.30 =

Magnifica-

maximum aperture, 0.60 = minimum aperture),

tion colour code, 5 Knurled ring for adjusting the correction

mount, 6 Knurled ring for adjusting the built-in diaphragm

Download from Www.Somanuals.com. All Manuals Search And Download.

Binocular tube HCI B22 or HCI BV22

For eyepieces with inserted graticule only*:

● Greatly defocus the specimen or remove

from the light path.

● Exactly focus the graticule by adjusting the

eyelens with a relaxed eye (the eye relaxes

best if you look out the window at a distant

object for a moment).

Eyeglasses with multirange lenses (bifocal and

progressive) must be removed for microscopy.

● Focus the specimen through the eyepieces.

Only when one eyepiece is without an

adjustable eyelens:

● Exactly focus the specimen through this eye-

piece first (close your other eye).

● Then focus the image by adjusting the eye-

lens of the second eyepiece.

● Focus the specimen, only adjusting the

eyepiece with graticule.

● Then close this eye and focus the specimen

by adjusting the second eyepiece only.

Only if neither eyepiece has a graticule inserted:

● Greatly defocus the specimen or remove it

from the light path.

● Adjust the eyelens until the edge of the field

of view appears sharp. When you adjust the

eyelens a white line becomes visible round

the basic part of the eyepiece. This indicates

the correct position of the eyelens for

viewers with normal or corrected eyesight.

Fig. 57 HCI B22 binocular tube, 45° viewing angle, field of

view no. up to 22, eyepiece diameter 30 mm for HC PLAN 10x/20

or 22 eyepieces, interpupillary distance setting: 55 – 75 mm

1 Eyepiece tube, 2 Eyepiece, 3 Anti-glare protection

Download from Www.Somanuals.com. All Manuals Search And Download.

To correct defective eyesight:

Trinocular tube HCI 3T22

● Look through the right-hand eyepiece tube ● Set the beamsplitter at visual observation by

with your right eye and sharply focus the

specimen with the fine drive.

● Then look at the same area of the specimen

with your left eye and rotate the left eyepiece

tube until you obtain a sharp image. Do not

use the fine drive for this.

pushing in the switch rod. The switching

positions are indicated by symbols on the

side of the tube.

100 % vis

– switch rod

150 % – 50 % – switch rod

100 % – photo – switch rod

● If using eyepieces with adjustable eyelenses, ● The eyepieces are set in exactly the same

do not compensate for defective eyesight by way as on the binocular tube.

adjusting the eyepiece tube, but by adjusting ● Compensate defective eyesight by adjusting

the eyelens of the eyepiece.

Fig. 59 HCI 3T22, trinocular tube with 45° viewing angle

Light path: 100 % vis

– switch rod

150 % – 50 % – switch rod

Fig. 58

100 % – photo – switch rod

HCI BV22, ergo binocular tube with 15° – 50° viewing angle,

field of view no. up to 22, eyepiece diameter 30 mm for

HC PLAN 10x/20 or 22 eyepieces, interpupillary distance set-

ting: 55 – 75 mm

Field of view no. up to 22, eyepiece diameter 30 mm for

HC PLAN 10x/20 or 22 eyepieces, interpupillary distance set-

ting: 55 – 75 mm

1 Clamp screw, 2 Tube port, 3 Siedentopf binocular part,

4 Photo/TV port, 5 Switch rod

1 Clamp screw, 2 Tube port, 3 Siedentopf binocular part

Download from Www.Somanuals.com. All Manuals Search And Download.

Operation of the side photo/TV port

Operation of the front photo/TV port

The delivery comprises two alternative outfits Stands either with or without SLR front port can

for the lateral photo/TV exit (Fig. 59a).

One outfit has a beam split of

be supplied.

The beam split is as follows:

100% visual 80 % side

120 % visual 80 % side

The side port is switched off, i. e. 100 % of the

light goes to the visual light path:

The second version has a beam split of

100 % visual 110 % side

110 % visual 100 % side

If the switch rod (60.3) for the SLR exit is pulled,

50 % of the light goes to the SLR and 50 % to the

tube.

If the switch rod (60.2) for the side port is pulled

out, the beam split version no. 2 is active. If the

switch rod is pushed in, beam split no. 1 applies.

Fig. 60 Bertrand lens engaged

1 Lever for focusing the Bertrand lens, 2 Switch lever for side

port, 3 Switch lever for front port

Download from Www.Somanuals.com. All Manuals Search And Download.

Operation of objectives

Immersion objectives

n. b.:

OIL: Only use DIN/ISO standard immersion oil.

When using the immersion objective again,

remember to release the lock, as otherwise the

spring mechanism that protects the specimen

and objective will not work and the other

objectives will no longer be parfocal with the

immersion objective.

n. b.:

Observe the safety information on the im-

mersion oil!

CORR objectives

W: Water immersion. The special water im-

mersion objectives with ceramic front part can

be used for all hydrous solutions.

IMM: Universal objective for water, glycerine

and oil.

These are special objectives which can be

adjusted to the thickness of the coverslip.

● Roughly set the correction mount to a medium

or estimated value by turning the knurl.

● Focus the specimen.

● Adjust the correction mount until you obtain

obtimum contrast, fine-tune the focus with

the fine drive if necessary. This setting may be

very difficult for featureless or low-contrast

areas of the specimen.

Colour coding of objectives

→ “Technical data”.

Locking objectives

Some immersion objectives (with knurled grip)

can be locked in a shorter position. This pre-

vents any remaining drops of immersion liquid

from wetting other objectives or specimens when

the nosepiece is turned.

● Press up the front part by about 2 mm.

● Lock the objective in this position by rotating

slightly.

Download from Www.Somanuals.com. All Manuals Search And Download.

Operation of transmitted light

Brightfield illumination

Setting the aperture diaphragm

Illumination techniques where the empty areas The aperture diaphragm determines the lateral

of the specimen are the brightest parts are resolution, field depth and contrast of the

called brightfield. Absorbing specimen struc- microscope image. The best contrast is

tures are required for brightfield imaging, i. e. obtained when the apertures of the objective

most specimens will need staining. Alternati- and the condenser are roughly the same.

ves are optical contrasting techniques such as When the aperture diaphragm is stopped down

phase or modulation contrast.

to be smaller than the objective aperture,

resolving power is reduced, but the contrast is

enhanced. A noticeable reduction in the re-

solving power is observed when the aperture

diaphragm is stopped down to less than 0.6x of

the objective aperture and should be avoided

where possible.

Setting the condenser

On the TL illumination column there are height

markings – S70, S23 and S1 – (13.3) for setting

the correct condenser height. Using the

supplied hexagonal screwdriver, slacken the

screw (14.1) and adjust the height of the

condenser or condenser holder until its upper

edge coincides with the corresponding con-

denser height marking on the illumination

column. Retighten the condenser or condenser

holder fixing screw.

Brightfield illumination with condenser

0.30 S70

Brightfield illumination is possible with objective

magnifications of 2.5x to 40x.

Turn a 10x objective into the light path and focus

the specimen with the coarse and fine drive.

Narrow the aperture diaphragm until you obtain

the desired image contrast.

Brightfield illumination with condensers

0.53 S23 and 0.90 S1

Brightfield illumination is possible with condens-

er 0.53 S23 with objective magnifications from 5x

to 100x, and with condenser 0.90 S1 from 10x to

100x. A P 1.40 OIL S1 condenser top is available

for extremely high resolution.

Download from Www.Somanuals.com. All Manuals Search And Download.

Setting Koehler illumination

Therefore it is only opened wide enough to

just illuminate the observed or photographed

object field. A change in magnification al-

ways necessitates adjustment of the field

diaphragm.

Turn a 10x objective into the light path and focus

the specimen.

– Engage the condenser disc into the “H” =

Hellfeld = brightfield position if necessary.

– Close the field diaphragm.

– Adjust the height of the condenser until the

edge of the field diaphragm is sharply in focus

and also:

– Narrow the aperture diaphragm until you

obtain the desired image contrast.

Centre the image of the field diaphragm in the

middle of the field of view with the two cen-

tring screws.

Open the field diaphragm until it just dis-

appears from the field of view.

When objectives are changed, the condenser

centration may have to be slightly adjusted

with the knurled screws and the field dia-

phragm reset.

The aperture diaphragm determines the lateral

resolution, field depth and contrast of the

microscope image. The best contrast is

obtained when the apertures of the objective

and the condenser are roughly the same.

The field diaphragm protects the specimen

against unnecessary heat and keeps all light

not required for imaging away from the

specimen, thereby enhancing contrast.

Fig. 62

1 Binocular observation and phototube, 2 Tube clamp screw,

3 Objective nosepiece, sextuple, 4 Clamp screw for SLR/TV

adapter, front port, 5 Transmitted light lamphousing, 6 Trans-

mitted light illumination column, 7 Condenser holder, 8 Con-

denser 0.53 S23 with disc, 9 Screw for opening lamphousing

105, 10 Lamphousing 105, 11 Adjustment wheel for tube lens

1x, 1.5x or Bertrand lens (B), 12 Beamsplitter switch rods,

13 Coarse and fine focus

Fig. 61 Koehler illumination

a Field diaphragm closed, not focused, not centred, b Field

diaphragm focused, but not centred,

focused and centred, but diameter too small, d Diameter of

Field diaphragm

field diaphragm

illumination)

diameter of field of view (Koehler

Download from Www.Somanuals.com. All Manuals Search And Download.

Visual comparison of the objective and con-

denser apertures is done as follows: remove

an eyepiece from the eyepiece tube, or engage

the Bertrand lens by turning the knurled wheel

(62.11), (pos. B) and focus with the lever (62.11).

Close or open the aperture diaphragm until the

image just shows up in the pupil (= brighter

circle) of the objective. This is regarded as the

standard setting, i. e. condenser aperture =

objective aperture.

n. b.:

The aperture diaphragm in the illumination

light path is not for adjusting image intensity.

Only use the brightness adjustment knob or

neutral density filters for this.

An aperture diaphragm in the objective is nor-

mally opened fully. Narrowing it reduces the

intensity and

Replace the eyepiece or disengage the Bertrand

lens.

increases field depth

reduces coverslip sensitivity

creates a darkfield impression

alters contrast

For low-contrast specimens, the aperture dia-

phragm can be narrowed further for clearer

imaging of fainter structures. In polarisation mi-

croscopy, narrowing the aperture diaphragm

usually results in stronger colours.

Possible errors

Wrong coverslip thickness or wrong objective.

Specimen with coverslip at the top instead of

the bottom.

Aperture diaphragm opened too far or closed.

Condenser at wrong height.

Light ring switched in by mistake.

Dirty optics.

Download from Www.Somanuals.com. All Manuals Search And Download.

Operation of phase contrast

Phase contrast observation

Set the light ring (64.2) in the condenser disc

that corresponds to the objective engraving

(PH2). Open the aperture diaphragm (= pos. PH).

Move the Bertrand lens into the light path =

pos. B by turning the knurled knob and focus the

annular structures with the lever (Fig. 64).

Like transmitted light darkfield and transmitted

light interference contrast, phase contrast is

used to produce high-contrast images of un-

stained specimens.

Setting phase contrast with condenser 0.30 S70

Insert the two supplied centring keys into the

openings of the disc on the left and right of the

label plate (e.g. 3) (Fig. 64.2) and turn them until

the dark ring (phase ring in the objective)

coincides with the slightly narrower ring (light

ring in condenser).

Phase contrast observation is possible with ob-

jective magnifications from 5x to 40x.

Turn a phase contrast objective (engraving e. g.

PH2) of the lowest magnification into the light

path and focus the specimen. If it is difficult to

find the focal plane: temporarily narrow the

aperture diaphragm or use a stained specimen

and switch the disc to pos. H (= brightfield).

Then repeat the centration process for the other

objective/light ring combinations. Disengage the

Bertrand lens, pos. 1x.

Fig. 63 Centration for phase contrast, viewing with a Bertrand

lens

a Condenser in brightfield position (H), b Condenser in PH

position, light ring LR not centred, c Light ring and phase ring

centred

Fig. 64 Centration process for phase contrast/DF

1 Centring keys in working position, 2 Disc

Download from Www.Somanuals.com. All Manuals Search And Download.

Setting phase contrast with

Possible errors

condensers 0.53 S23 and 0.90 S1

Specimen: too thick, too thin, staining too

Phase contrast observation is possible with intense; refractive index of mounting medium

condenser 0.53 S23 with objective magnifica- and specimen identical, so there is no phase

tions from 5x to 100x, with condenser 0.90 S1 jump.

from 10x to 100x.

Specimen slide too thick, so Koehler illumination

For both condensers, phase contrast is set as not possible.

described as for the 0.30 S70 condenser.

However, before the centration process itself,

correct Koehler illumination must be set.

Wedge-shaped coverglass position, so centra-

tion of light and phase ring is no longer effective.

Wrong light ring, or light ring has been put in the

disc upside down.

Aperture diaphragm not open.

Light ring not centred.

Wrong light ring.

Download from Www.Somanuals.com. All Manuals Search And Download.

Operation of transmitted light darkfield

Darkfield observation

Rotate the condenser disc to the H position

(= brightfield). Focus the specimen (5x/10x

objective). If the specimen plane is difficult to

find, temporarily close the aperture diaphragm.

Set Koehler illumination, open the aperture

diaphragm as far as the stop (= pos. PH) and

turn the disc to position D (= darkfield dia-

phragm).

Darkfield observation is not possible with

condenser 0.30 S70, with condenser 0.53 S23 it is

possible from 5x objective magnification, the

max. usable objective aperture is 0.40. With

condenser 0.90 S1, DF observation is possible

from objective magnification 10x, the max.

usable objective aperture is 0.75.

If the specimen does not appear against a dark

background, centre the DF diaphragm with the

centring keys. To do this, insert them in the

openings in the disc on the left and right of the

label plate for the DF diaphragm (D) (64.2) and

rotate until a homogeneous dark specimen

background is produced.

Objectives with higher apertures can be used if

it is possible to reduce the aperture with a built-

in iris diaphragm. These objectives can be

recognised by the fact that the maximum and

minimum apertures are given in the objective

engraving and in our lists, e. g. 1.30 – 0.60.

Download from Www.Somanuals.com. All Manuals Search And Download.

Operation of transmitted light polarisation

DL polarisation

Push the analyser into the 2nd clickstop position

in the microscope with the engraving ICT facing

upwards.

Polarisation contrast for examining birefringent

specimens is possible with condenser 0.30 S70

with objective magnifications from 2.5x to 40x,

with condensers 0.53 S23 or 0.90 S1 from 5x or

10x to 100x. A P 1.40 OIL S1 condenser top is also

available for extremely hgh resolution.

Set the optimum extinction position by rotating

the polariser and watching the empty field of

view. Put a specimen on the stage.

For Pol colour contrast, the ICT analyser can be

turned over, with the lambda engraving facing

upwards, to activate a whole-wave compensator.

Crossing the polarisers

First: Set Koehler illumination. Remove the speci-

men from the light path; remove the Bertrand

lens and fluorescence filter cube if necessary;

turn the condenser disc and turret for objective-

side IC prisms to pos. H.

Insert the polariser into the filter holder with the

engraving facing upwards. Turn the filter holder

to the right into the light path.

Download from Www.Somanuals.com. All Manuals Search And Download.

Operation

of transmitted light interference contrast

TL interference contrast

Insert a polariser into the filter holder with the

engraving facing upwards.

Turn the filter holder to the right into the light

path.

Push the analyser into the 2nd clickstop position

in the microscope with the engraving ICT facing

upwards.

TL interference contrast observation is possible

with condenser 0.30 S70 with objective mag-

nifications from 10x to 40x, with condensers

0.53 S23 or 0.90 S1 from 10x to 100x. For

objective 100x there is also a condenser top

P 1.40 OIL S1 for extremely high resolution.

Set the optimum extinction position by rotating

the polariser and watching the empty field of

view.

Crossing the polarisers

Remove the Bertrand lens and fluorescence

filter cube from the light path if necessary; turn

the condenser disc and turret for objective-side

IC prisms to pos. H. Focus the specimen (20x

objective). Set Koehler illumination exactly (not

needed for condenser 0.30 S70). Remove the

specimen from the light path.

Download from Www.Somanuals.com. All Manuals Search And Download.

Centration of the condenser prisms

Objectives for ICT

If you have ordered a complete microscope, this Transmitted light interference contrast is

adjustment will already have been made at the possible with the brightfield and phase contrast

factory. However, it is advisable to check the objectives which have the code letter of the

centration from time to time, particularly after pupil position in the first line of engraving, e. g. A

transport: disengage the objective-side IC (see separate objective chart).

prisms (pos. H).

An IC condenser prism, e. g. K6, must also be

available for the objective. An up-to-date table

Remove an eyepiece from the eyepiece tube. of possible prism combinations (objective chart)

Engage the condenser-side IC prisms one after is enclosed separately with each configuration.

the other (the whole-wave compensator must

not be active, i. e. the lambda engraving is on the

bottom side of the analyser). When the cen-

tration is correct, the dark stripe must be in

the centre of the pupil (= brighter circle) of the

objective (Fig. 65).

If not, proceed as follows:

Put one of the supplied centring keys in the disc

opening on the left of the label plate for the IC

prism (e. g. 64.2) (Fig. 65) and turn it to centre the

stripe.

Fig. 65

Fig. 66 Centration for interference contrast

Objective pupil with correctly centred compensation stripe

1 Centring keys in working position

Download from Www.Somanuals.com. All Manuals Search And Download.

Choice of prisms

Sources of error if ICT image quality

is unsatisfactory

Choose the objective-side prism with the letter

indicated in the top line of the objective en- Embedding medium, specimen slide (petri dish)

graving, e. g. C for pupil position C, by rotating or specimens (e. g. crystals, fibres) are of

the turret.

birefringent material. The phase shifts caused

by birefringence disturb the interference con-

Choose the condenser-side prism that corre- trast image. This can sometimes be remedied

sponds to the magnification of the objective by rotating the specimen.

used, e. g. pos. 40 for objective 40x, by rotating

the disc.

Setting ICT contrast

Turn the objective-side prism turret to the left

and right (Fig. 67). Also adjust the contrast with

the aperture diaphragm. Optimum contrast for

specimens with parallel structures can be

obtained by rotating the specimen. Colour

contrast: Turn over the analyser, so that the

lambda sign can be seen on the top.

Fig. 67

Setting ICT contrast

Download from Www.Somanuals.com. All Manuals Search And Download.

Operation of incident light fluorescence

The BG 38 filter should always be used for

photography.

n. b.:

When not looking through the microscope,

always block the incident light path to prevent

specimens fading. Push the switch rod in all the

way.

Only with microscope with integrated incident

light fluorescence axis.

Fluorescence observation

The 3 clickstop positions of the switch rod mean:

Switch rod

Focus the specimen in transmitted light first, if

possible (perhaps Phaco or ICT).

Select a filter cube to suit the excitation and

emission spectrum of the specimen and move

into the light path by rotating the turret. Open the

iris diaphragm of the objective.

Switch the magnification changer* (on the

DM IRB-SLR and DM IRBE versions) by turning

the knurl to pos. 1x. Switch off the transmitted

light illumination.

Stop ⅷ

Incident light path blocked

(light stop)

BG filter engaged

BG 38

⅜

Incident light path open

Open the incident light path. The switch rod

should be pulled out fully.

If the background is too red, engage a BG 38

filter. Push the switch rod in halfway.

Download from Www.Somanuals.com. All Manuals Search And Download.

Method 2:

Centration of the 12 V 100 W, Hg, Xe lamps

Centration in the rear focal plane of the

objective

Lamphousing 107/2 for 12 V 100 W halogen lamp

This lamphousing is permanently set and does

not require centration. However, it is essential

that the lamp is aligned straight in its mount.

1. Turn a low-power objective into the light path

and, using the BF reflector, focus on a

strongly reflecting specimen (e. g. surface

mirror) with the coarse and fine drive. Open

the field and aperture diaphragm (72.1 + 72.3).

2. Remove the eyepiece from the right or left

tube and look into the empty eyepiece tube.

3. Slightly reduce the light intensity until the

back objective pupil (back lens surface of the

objective) can be clearly seen.

Lamphousing 107 L for 12 V 100 W halogen lamp

(Fig. 68)

3 alternative centration methods:

Method 1:

Centration with a centring aid

On the right side of the microscope there is an 4. Adjust the lamp collector (68.4) until you see

adjustment window showing an image of the

light source. The reflector for lamp adjustment is

inserted in the filter turret instead of a filter cube

and turned into the light path.

the structure of the lamp filament. The

filament image is divided into two with a pale

stripe in the middle (Fig. 69).

Please note that only the central area of the

filament can be seen and that the image is

very low in contrast.

Centre the lamp as described for method 2 while

watching the light source in the adjustment

window.

Fig. 69 Lamphousing 107/2 and 107 L

Reflection of the lamp filament (greatly schematized): the

reflection is actually very low in contrast, the pale overlap

area is wider and more blurred. For lamphousing 106 z the

reflection is rotated by 90°.

Fig. 68 Lamphousing 107 L

1 Cover fixing screws, 2 Screw for horizontal adjustment,

3 Screw for vertical adjustment, 4 Collector focusing

Download from Www.Somanuals.com. All Manuals Search And Download.

5. Using an Allen key, adjust the screw for hori- 5. Using the centring screws, slide the image of

zontal adjustment (68.3) until the pale stripe

of the filament image is in the centre of the

pupil.

the filament into the middle of the centration

area marked with a dot or cross, as described

in Method 2.

6. Then adjust the screw for vertical adjustment

(68.2) to align the filament image vertically in Lamphousing 106 z L with halogen lamp,

the centre of the pupil.

Xe and Hg lamps

(switch gas discharge lamps on and off at sepa-

rate power units)

Method 3:

Centration in the plane of the specimen stage

1. Put a piece of paper or non-shiny piece of For lamphousing 106 z the direct lamp image and

Leica packaging on the specimen stage and the reflection of the reflector are focused

roughly focus the surface with a low-mag- separately and aligned to each other.

nification objective.

Either of the above methods can be used for

2. Set the field and aperture diaphragms at the imaging the lamp filament or arc.

middle position.

3. Make a dot or cross on the centration area Centration of 12 V 100 W halogen lamp

with a felt or ball point pen and slide it into

Move the reflection of the filament to the side or

the centre of the spot of light. Fix with the

entirely out of the light path by adjusting the

specimen clip if necessary.

centring screws on the back of the lamphousing

4. Screw out the objective or turn an empty

(70.5, 71). Focus the direct image of the filament

nosepiece position into the light path.

with the collector adjustment (70.1)

Then, using the centring buttons, adjust the

image of the filament until the centration area or

rear focal plane of the objective is half filled

(Fig. 71b).

Fig. 70 Lamphousing 106 z with Hg 100 W lamp

1 Lamp adjustment, vertical, 2 Reflector adjustment, vertical,

3 Focusing of the reflector image, 4 Reflector adjustment, ho-

rizontal, 5 Lamp adjustment, horizontal, 6 Collector focusing,

7 Cover fixing screw

Then focus the reflection of the filament with the

centring buttons for the reflector adjustment

and align symmetrically to the direct image

(Fig. 71c).

Risk of glare with gas discharge lamps! Use

neutral density filter (see p. 56).

Download from Www.Somanuals.com. All Manuals Search And Download.

Hg 100 W and Xe 75 W lamps

n. b.:

Using the centring buttons (70.1, 70.5) move the

direct image of the arc to the middle of the

centration area, with the bright tip of the arc, the

focal spot of the cathode, just off centre.

Then focus the reflection (70.3) and, using the

centring buttons of the reflector adjustment,

move the reflection until it is symmetrical with

the direct image (Fig. 71a, b, c).

Never look straight into the light path!

Remember the risk of glare when switching

to the BF or Smith reflector!

Centration of Xe or Hg gas discharge lamps

Move the reflection of the discharge arc to the

side or entirely out of the light path by adjusting

the centring screws on the back of the lamp-

housing (70.2, 70.3, 70.4).

The V-shaped emissions of the arcs of the direct

image and the reflection can be superimposed.

Focus the direct image of the arc with the

collector adjustment (70.6).

Caution:

The bright tip of the light arcs, the focal spots

of the cathode, must never be projected on

top of one another, as there is then a risk of

explosion due to overheating.

Replace spent burners in good time and

dispose of in an environmentally compatible

way.

Open lamphousing only after cooling and

disconnection from the mains.

Wear gloves and mask if using Xe lamps.

Hg lamps will reach their full intensity only

after a few minutes, they do not ignite when

hot.

Caution:

Use the neutral density filter to reduce the

intensity of the discharge arc image on the

centration areas due to the risk of glare

damaging the eyes.

Centre the arc images as follows:

Hg 50 W mercury lamp

Using the centring buttons (70.1, 70.5) move the

direct image of the arc to the right or left of an

imaginary line through the middle of the

centration area. Then focus the reflection (70.3)

and, using the centring buttons of the mirror

adjustment (70.2, 70.4), move the reflection until

it is symmetrical with the direct image (Fig. 71c).

Download from Www.Somanuals.com. All Manuals Search And Download.

Fig. 71

Schematic diagram of the lamp centration in lamphousing 106 z (in reality the lamp images are not as sharp)

a direct lamp image, focused, but decentred

b direct lamp image in correct position

c indirect and direct lamp image in correct position

Halogen

lamp

Hg 50

lamp

Hg 100 /

Xe 75

lamp

Download from Www.Somanuals.com. All Manuals Search And Download.

Centring the aperture diaphragm

Centring the field diaphragm

Turn a low to medium objective magnification Turn a low to medium objective magnification

10x/20x into the light path and focus a specimen 10x/20x into the light path and focus a specimen

with the coarse and fine drive.

Open the field diaphragm almost as far as the

Remove an eyepiece from one of the two edge of the field of view.

eyepiece tubes and look into the empty tube or Using the centring buttons (72.4), centre the field

move the Bertrand lens into the light path.

diaphragm to the edge of the field of view.

Regulate the light intensity so that the rear

objective pupil (rear lens surface of the ob- The field diaphragm is imaged on the surface of

jective) can be clearly seen.

the specimen, framing the illuminated field.

Normally, the field diaphragm is opened until it

Using the adjustment button (72.1), open the just disappears out of the field of view.

aperture diaphragm nearly to the edge of the When imaging reduced picture diagonals such

pupil.

as in photomicrography or TV microscopy, the

Centre the aperture diaphragm to the edge of field diaphragm can be narrowed to frame the

the pupil with the centring screws (72.2).

picture format, enhancing the image contrast.

The aperture diameter of the field diaphragm

The aperture diaphragm influences the reso- remains the same for all objective magnifi-

lution, contrast and field depth of the micro- cations.

scope image. Image quality greatly depends on

how carefully it is set. It may not be used for

regulating the image intensity.

Fig. 72 Aperture and field diaphragm

Aperture diaphragm adjustment,

Aperture diaphragm

Field

centring screws, Field diaphragm adjustment,

diaphragm centring screws

Download from Www.Somanuals.com. All Manuals Search And Download.

Possible errors

Low-contrast image due to:

Excitation bandwidth too wide.

Inspecific staining.

Weak fluorescence, insufficient brightness:

Wrongly stored, overaged or faded specimens.

Fast fading of the specimens (e. g. for FITC).

Unspecified filter combination.

Numerical aperture of the objective too low.

Eyepiece magnification too high.

Spent lamp.

Fluorescing mounting medium.

Autofluorescence of objective or immersion oil.

Glass surfaces dirty.

Room too bright.

Trinocular tube: wrong beamsplitter setting.

Stray light due to reflections at the condenser.

Download from Www.Somanuals.com. All Manuals Search And Download.

Operation of filters

Light filters

Up to max. 3 light filters can be inserted in the filter holder (1.16). They can be switched in and out

the light path as required.

Filter

Use

Grey filter

Grey filters (neutral density filters) are used to

attenuate the light without influencing the

colour temperature. The engraved value, e. g.

N16, indicates the attentuation value. So N16

means reduction to 1/16 = 6.3 % transmission.

Green filter, panchromatic

for general enhancement of contrast in black-

and-white photography.

DLF

Conversion filter for colour photography with

daylight film.

ALF

Enhances contrast for colour photography with

artificial light film.

VG9 (green filter )

Contrast enhancement for chromosome photo-

graphy.

Download from Www.Somanuals.com. All Manuals Search And Download.

Operation of the slide overlay device

Slide overlay device

The original is imaged 2 : 1 in the intermediate

image plane of the microscope. A distance of

e. g. 5 mm in the slide overlay is enlarged to

10 mm in the intermediate image plane of the

microscope.

The slide overlay device is used for reflecting

measurement and comparison patterns, µm

marks, marker arrow, company logo and quality

data etc. into the microscope image so they

appear on the photograph.

The overlay is only possible in beamsplitter

position 50/50 (switch rod) in the middle position

of the tube (FSA 25 PE).

Slides with the following line patterns are

available:

Marker arrow

Measurement

scale 10 mm = 100 divisions

µm marks for 2.5x – 100x objectives

10 x 10 mm grid division

The framed slide is inserted in the fitted slide

holder (74.6), with the lettering on the white side

of the slide facing the lamp.

The slide holder can be adjusted on all sides, so

that the overlay can be positioned anywhere in

the microscope image. Remember that when

you move the slide, the overlay in the image will

move in the opposite direction. This takes a bit

of getting used to.

You can make your own masks with any meas-

urement and comparison line patterns, quality

data, company logos, etc.

The original master has to be copied on a 35 mm

negative, i. e. white line patterns on a dark back-

ground, preferably using fine-grain document

film, and then framed in a customary 50 x 50 mm

slide frame.

The white line pattern can be given a coloured

background by inserting 32 mm colour filters in

the filter slot (74.7).

Download from Www.Somanuals.com. All Manuals Search And Download.

Operation of the macro device

Like the slide overlay device, the macro overlay Stand lamps, cold-light illuminators and fibre-

(Fig. 73) only works in the 50/50 beamsplitter optic lamps, etc. are suitable sources for micro-

position (switch rod in middle position) of the scopy.

FSA 25 PE tube.

The microscope illumination is left switched off and focused by turning the knurled ring (73.10).

to avoid disturbing image brightening. The magnification can be changed continuously

The image is observed in the microscope tube

The object is placed on the stage under the in a range of 1 : 4 by adjusting the zoom ring

mirror housing of the macrodual zoom (73.11) (73.7).

and illuminated.

When changing the magnification with the zoom

control the image has to be slightly refocused

with the knurled ring (73.10). The zoom

magnification factors can be read on the scale

(73.8). The magnification also changes when the

distance between the object and the macro

attachment is varied.

Fig. 73 Macro device on FSA 25 PE tube with tube adapter

1 Tube flange, 2 Coupling ring, 3 Reflection optics, 4 Coupling

ring, 5 Macro adapter, 6 Screw ring, 7 Zoom setting ring 1 : 4,

8 Scale of zoom factor, 9 Scale of magnification factor of the

working distance, 10 Scale of object distance from the bottom

edge of the mirror housing, 11 Mirror housing

7 9

1 2 3

10 11

Download from Www.Somanuals.com. All Manuals Search And Download.

The total magnification in the microscope, the Viewed with a 10x eyepiece, this intermediate

reproduction ratio on the photograph or TV image of 0.1x gives a total magnification of 1x in

image can be quickly and easily measured with the microscope eyepiece (0.1 x 10 = 1x).

a scale and calculated.

The total magnification of the film plane of a

n.b.: For normal viewing without the macro camera is derived from multiplying the inter-

mirrorhousing or macrodual zoom, put on the mediate image magnification M₁by the

cover to avoid disturbing overlay effects.

magnifications of the photo eyepiece and

camera attachment, e. g.:

The mirror housing (73.11) can be rotated intermediate image magnification 0.1x

through 360°, for example to alter the angle at photo projection lens 10x

which the photograph is taken. This is done by camera factor 35 mm 0.32x

loosening the Allen screw.

0.1 x 10x1.32 = 0.32x

The total magnification at the 35 mm camera of

The intermediate image magnification M₁of the the ORTHOMAT^®E is therefore 0.32x.

macro object can be worked out from the

eyepiece field of view and the diameter of the

object field (measured with a graduated ruler)

as follows:

z. B.

field of view Ø

10x/20 eyepiece

M₁= –––––––––––– e. g. –––––––––––––––––– M = 0.1

object field Ø object field = 200 mm

Fig. 74

Slide overlay on the FSA 25 PE tube (with tube adapter)

1 Tube flange, 2 Coupling ring of reflection optics, 3 Reflection

optics, 4 Coupling ring of slide overlay device, 5 Knurled

focusing ring, 6 5 x 5 cm slide holder, 7 Filter slot, 8 Illumina-

tion adapter of lamphousing

Fig. 75 Transformer

Download from Www.Somanuals.com. All Manuals Search And Download.

The total magnification can be roughly Use of the macrodual zoom as a drawing device

calculated with the scale divisions on the Drawing microstructures under the microscope

macrodual zoom:

has the advantage over photomicrography that

significant details can be highlighted and that

The following factors have to be multiplied for structures can be depicted in three dimensions.

this: This is not possible with photomicrography.

– Magnification factor of the working distance Apart from this, drawing with the superimposed

(scale (73.9), e. g. 0.11x)

– Zoom factor (scale (73.8), e. g. 1x)

image method is a valuable didactic exercise.

It is done by superimposing the drawing area

– Correction factor of the reflection optics (the area of the stage under the mirror housing

(without engraving 1.17x)

– Eyepiece magnification (e. g. 10x)

e. g. 0.11 x 1 x 1.17 x 10 = 1.29

of the macrodual zoom) onto the microscope

image. The drawing area or sheet of paper is

homogeneously illuminated with a stand lamp or

table lamp.

The total magnification in the eyepiece would The microscope illumination and illumination of

therefore be 1.29x.

the drawing area are matched providing the

lamps are adjustable; otherwise the brightness

of the drawing area can be varied by altering the

proximity of the lamp.

Download from Www.Somanuals.com. All Manuals Search And Download.

Length measurements

Important: If using a magnification changer*

(DM IRB-SLR and DM IRB/E stands):

The following components are required for

length measurements:

– Graticule with scale in eyepiece (Fig. 76) or in

the slide overlay device (Fig. 74).

– Transmitted light stage micrometer for cali-

bration.

Remember to take the additional magnification

value into consideration separately instead of

extrapolating the micrometer values of the other

objectives from the calibration of one objective.

Measurement errors may occur if the eyepiece

is not pushed into the tube as far as the stop.

Before measurement, the micrometer value of

the objective/eyepiece combination must be

known, i. e. the distance in the specimen that

Connections for TV cameras and

photomicro equipment

corresponds to a scale interval in the graticule All the variants of the Leica DM IRB stand have

you are using.

a photo/TV exit on the left side.

There are also photo/TV exits in the trinocular

tubes for vertical adaption of camera systems.

Calibration:

Align the stage micrometer and the graticule

parallel to one another by rotating the eyepiece

and adjust the zero marks of the two scales to

exactly the same height (Fig. 76).

Read how many scale divisions of the stage

micrometer correspond to how many on the

microscope scale (graticule) and divide the two

values.

Example:

If 1.220 mm of the stage micrometer corre-

sponds to 100 divisions of the measurement

scale, the micrometer value is = 1.220 : 100 =

Fig. 76 Graticule scale in the eyepiece (left) and image of the

stage micrometer (right)

0.0122 mm

12.2 µm. For extremely low

objective magnifications it may be that only part

of the measurement scale can be used for

calibration.

Download from Www.Somanuals.com. All Manuals Search And Download.

Various adapters are available for connecting TV cameras with c-mount or B-mount objective

thread:

Recorded picture diagonal in mm with

1 inch

²/₃inch

¹/₂inch

¹/ inch

Order no.

camera

Without zoom magnification, for 1 chip cameras

c-mount adapter 1x HC

c-mount adapter 0.63x HC⁺⁾

c-mount adapter 0.5x HC

c-mount adapter 0.35x HC

c-mount adapter 4x HC⁺⁾

–

17.5

–

2.8

12.7

–

6.5

9.5

12.5

17.1

1.5

541 510

541 537

541 511

541 512

Without zoom magnification, for 1 – 3 chip cameras

each in connection with 0.5x HC TV optics (screw connection

)

c-mount adapter 1x

B-mount adapter 1x

B-mount adapter 1.25x

F-mount adapter 1x

F-mount adapter 1.25x

–

17.5

–

12.5

–.5

12.5

–.5

541 706

541 702

541 539

541 540

541 541

541 706

17.5

required for each: TV adapter 0.5x HC

With zoom magnification (Vario TV adapter)

c-mount, 0.32 – 1.6x HC

B-mount, 0.5 – 2.4x HC (SONY)

⁺⁾in preparation

–

19⁺⁺⁾– 5

16 – 3.3

18 – 3.8

–.5

541 517

541 518

⁺⁺⁾from zoom factor 0.42x only!

Fig. 77 C-mount adapter on side port

1 TV camera, 2 Adapter with c-mount thread (or B-mount

bayonet), 3 Clamp screw, 4 Photo adapter tube

2 4

Download from Www.Somanuals.com. All Manuals Search And Download.

Calculation of the magnification on the monitor DM IRB with side photo port and front port

(DM IRB-SLR) and correspondingly two beam-

splitter switch rods (81.1 and 81.2).

For all TV exits the magnification on the monitor

can be calculated with the following formula:

– Image recording via phototube or side port:

M_TV= objective magnification x tube factor x

Use the upper switch rod (81.1) as for the

monitor diameter

x ––––––––––––––––––––

chip diameter of camera

version with side photo port only.

Push the lower switch rod (81.2) in (82.3 and

82.4).

adapter magnification

Beamsplitting for photomicrography

or TV microscopy

– Image recording via front port* (SLR/TV) or

phototube:

DM IRB with side photo port only and corre-

spondingly with one beamsplitter switch rod

(81.1).

Upper switch rod (81.1) pushed in, lower

switch rod (81.2) pulled out = 50 % light to the

front port and 50 % to the tube (82.5).

– Image recording via phototube:

Switch rod pushed in = 100 % light to the tube

(82.1).

– Image recording via side photo port:

Switch rod pulled out = 80 % light to the side

port and 20 % light to the tube (82.2).

Fig. 79 Adaption of the front port for TV camera

Fig. 78 Adaption of the front port for the SLR camera

1 TV adapter 0.63x, 2 TV camera with c-mount thread

1 SLR adapter, 2 T2 connector ring, 3 SLR camera

Download from Www.Somanuals.com. All Manuals Search And Download.

n. b.:

Long-term video microscopy

The solid and therefore stable basic body of the

stand takes a while to stabilise thermally after

the microscope is switched on. For investiga-

tions taking over > 30 min. therefore, the

microscope should be switched on about

1 – 2 hours beforehand.

Fig. 81 Switch rods for beamsplitting

1 Upper beamsplitter switch rod (SIDE), 2 Lower beamsplitter

switch rod (FRONT)

Fig. 80 Leica DM IRB, equipped with three TV cameras

Fig. 82 Beamsplitting

1 100 % light to the tube, 2 80 % light to the side photo port, 20 % to the tube, 3 100 % light to the tube, 4 80 % light to the side

photo port, 20 % to the tube, 5 50 % light to the front port, 50 % to the tube

SIDE

OFF

SIDE

OFF

SIDE

OFF

FRONT

OFF

FRONT

OFF

FRONT

Download from Www.Somanuals.com. All Manuals Search And Download.

Operation of LMC

Leica modulation contrast (LMC) is a special Further advantages of this technique are:

form of oblique illumination based on the

principle of Hoffmann modulation contrast.

– high contrast

– high resolution

In this technique, the phase gradients of an – halo-free, high-contrast relief image

unstained specimen are converted into dif- – long free working distance of the condenser

ferences in amplitude with the aid of a modu- – easy assembly and adjustment

lator.

–

use for both stained and unstained specimens.

This gives a three-dimensional impression simi-

lar to an interference contrast image. Unlike

interference contrast, however, the specimen

can be observed through birefringent plastic

materials such as petri dishes.

Download from Www.Somanuals.com. All Manuals Search And Download.

Principle of LMC

The principle

The light coming from the light slit diaphragm is

diffracted at the object into different directions,

depending on the object’s refractive index

gradient, so that some of the rays have to pass

through the light zone of the modulator and

some through the dark zone. The non-diffracted

direct light passes through the grey zone and

produces the grey background of the entire field

of view. Most of the rays diffracted at the object

pass through the light zone and produce the

image.

Leica modulation contrast (LMC) is based on the

principle of Hoffmann modulation contrast.

This imaging technique is particularly suitable

for unstained, colourless objects with little

image contrast.

Such objects change the phase of the light

when it passes through them.

The conversion of these phase gradients into

differences in amplitude results in a three-di-

mensional image similar to that of differential

interference contrast.

If the condenser is set at the “brightfield”

position and the specimen is removed, the dark

and the grey zone can be seen at the edge of the

field of view. The image of the slit diaphragm is

in the light zone. To adjust, the light slit

diaphragm is rotated until the bright stripe of the

slit image covers the grey stripe of the modu-

lator.

To realise this technique, a light slit diaphragm

and an objective with integrated modulator are

required. The modulator is a filter built into the

rear focal plane which divides it into three

zones, a dark zone, a grey zone and a light zone.

Download from Www.Somanuals.com. All Manuals Search And Download.

Components

The components

LMC objectives

LMC consists of the following components:

The following objectives are available:

S40/0.50 LMC condenser

C PLAN 10x/0.22 LMC

C PLAN L 20x/0.30 LMC

C PLAN L 40x/0.50 LMC

N PLAN L 20x/0.40 CORR LMC

N PLAN L 40x/0.55 CORR LMC

PL Fluotar L 63 x/0.70 CORR LMC

The condenser (order no. 521 225) is supplied

with a condenser disc to accommodate 3 LMC

diaphragms, plus two phase contrast light rings

and a brightfield position (3x LMC, PH1, PH2,

brightfield).

Adhesive labels are enclosed for labelling the

individual positions.

In the front focal plane of the condenser a light

slit diaphragm has already been assembled in

each of the LMC positions, corresponding to the

supplied objectives.

In the rear focal plane of the objectives a

special modulator, similar to the phase contrast

rings, has been fitted.

When unpacking, check that you have all the

components.

Download from Www.Somanuals.com. All Manuals Search And Download.

Assembly/adjustment

Assembly

Adjustment

When taking the following steps, consult the Open the aperture diaphragm on the condenser

manual for the Leica DM IRB/E manual.

fully.

Switch on the light. Select a medium brightness

setting.

n. b.:

Before installing the LMC components, remove

the field diaphragm. Also remove any filters,

prisms and interference contrast components.

Set the condenser to the brightfield position

and turn the first LMC objective into the light

path (usually the objective with the smallest

magnification).

Screw the LMC objectives into the objective

nosepiece.

Engage the Bertrand lens using the adjustment

wheel on the right side of the microscope.

Replace the condenser on the microscope with

the S40/0.50 LMC condenser. First check that the

inserted light slit diaphragms match the

objectives in the nosepiece. The light slit dia-

phragms are labelled, for example, LMC 10,

LMC 20 or LMC 40.

You will now see the modulator built into the

objective as a grey rectangle at the edge of the

field of view. Its position (top, bottom, left, right)

is not fixed and may vary for different objectives.

Focus the image of the modulator using the

Bertrand lens.

Example: LMC 10 diaphragms belong to the

C PLAN 10x/0.22 LMC objective.

Turn the condenser to switch to the light slit

diaphragm whose name corresponds to the

engraving on the objective (e. g. LMV 10 for the

C PLAN 10x objective).

The light diaphragms are usually assembled at

the factory to match the supplied objectives. If

the light slit diaphragms are supplied separately,

they must be inserted in the positions in the

condenser disc to match the objectives used.

You will now see a bright rectangle.

100

Download from Www.Somanuals.com. All Manuals Search And Download.

The light slit diaphragm is now adjusted until the Always make sure that the objective name and

bright stripe of the slit image is fully inside the the name of the light slit diaphragm coincide.

grey stripe of the modulator. The light slit

diaphragm can be rotated and moved in x and y Then disengage the Bertrand lens with the

direction.

adjustment wheel on the right side of the

For the 10x objective the image of the modulator microscope. Switch on magnification 1x or

and the light slit are virtually the same size. higher and put a specimen on the stage.

Adjust the light slit diaphragm until the bright slit

lies near the dark edge.

Repeat this process for each objective.

101

Download from Www.Somanuals.com. All Manuals Search And Download.

Areas of application

On the Leica DM IRB/E microscope, LMC is Avoidance of halo effects

particularly suitable for life science applica-

tions.

Phase contrast images are often spoiled by halo

effects. These do not occur with LMC.

Use of birefingent materials

Use for fluorescing specimens

Transparent, living cultures in petri dishes can

be observed in three dimensions, for example.

Morphology of fluorescing and non-fluorescing

specimens can be analysed without changing

the objective or moving the specimen.

Use of a micromanipulator

The long free working distance of the S40/0.50

LMC condenser offers plenty of space for

manipulation tools. The 3D image impression

makes it easier to find suitable injection points.

Optical sectioning

LMC produces a large, flat observation area.

This makes it easier to focus a specific area for

observation.

102

Download from Www.Somanuals.com. All Manuals Search And Download.

Care and maintenance

Cleaning of lacquered components

n. b.:

Dust and loose particles of dirt can be removed

with a soft brush or lint-free cotton cloth.

Before cleaning and maintenance work, re-

member to disconnect from the mains!

Protect electric components from damp!

Obstinate dirt can be removed with any ordinary

hydrous solution, benzine or alcohol.

Use a linen or leather cloth moistened with one

of these substances to clean lacquered compo-

nents.

Microscopes in warm and humid climates need

special care to keep them free of fungus.

The microscope should be cleaned every time it

is used and the microscope optics should be

kept immaculately clean.

n. b.:

Do not use acetone, xylol or nitro dilution, which

may damage the microscope.

Dust protection

Cleaning agents of unknown composition should

be tested on an inconspicuous part of the

microscope first. Lacquered or plastic surfaces

must not be tarnished or etched.

n. b.:

Protect the microscope and peripherals from

dust by putting on the flexible dust cover after

each work session.

Cleaning the stage

Remove light spots on the stage by rubbing with

paraffin oil or acid-free vaseline.

Cleaning

Cleaning glass surfaces

n. b.:

Remove dust on glass surfaces with a fine, dry

and grease-free hair brush, by blowing with a

bellows ball or by vacuum suction.

Fibre and dust residue can cause disturbing

background fluorescence in fluorescence micro-

scopy.

103

Download from Www.Somanuals.com. All Manuals Search And Download.

Obstinate dirt on glass surfaces can be carefully Removal of immersion oil

removed with a clean cloth moistened with

distilled water. If the dirt can still not be

removed, pure alcohol, chloroform or benzine

can be used instead of distilled water.

n. b.:

Read the safety information for immersion oil!

Cleaning objectives

First wipe the immersion oil off with a clean

cotton cloth and then wipe several times with

ethyl alcolhol.

n. b.:

Objectives should not be screwed apart for

cleaning. If there are signs of interior

damage, send the objectives to your nearest

Leica agency for repair. We also advise

against cleaning the inner surfaces of

eyepieces.

Acids, alkaline solutions

Particular care should be taken when working

with acids or other aggressive chemicals.

n. b.:

Always avoid direct contact between such

chemicals and the optics or stands.

The front lenses of objectives are cleaned as

described under “Cleaning glass surfaces”. The

top lens is cleaned by blowing off the dust with a

bellows ball.

104

Download from Www.Somanuals.com. All Manuals Search And Download.

Troubleshooting

All Leica instruments are manufactured and Electric errors

tested with extreme care. If you do have cause

for complaint, however, please do not try to

These may be:

repair the instruments and their accessories

1. The lamp on the microscope does not work.

2. There is no power.

yourself. Contact your national agency or our

central servicing department, the Technical

Service in Wetzlar, direct. Postal address:

Check the following possible causes:

Leica Microsystems Wetzlar GmbH

Abt. Technischer Service

Postfach 20 40

D-35530 Wetzlar

Tel. (0) 64 41-29 28 49

Fax (0) 64 41-29 22 66

The on/off switch does not respond

(no illumination):

● Check that all mains cables are properly con-

nected.

● Make sure that there is power at the sockets

you are using and that they are not

deactivated by a mains switch.

● After you have ruled out the possibility of all

possible external sources of error, check that

a fuse of the Leica DM IRB or power unit is not

defective.

Apart from preparation errors (e. g. staining or

wrong specimen vessel), which cannot be dealt

with in this manual, there are two main

categories of error:

Mechanical errors and

electric errors.

Replacing the mains fuse on the microscope

Mechanical errors

We already mentioned possible mechanical

errors in the “Installation” and ”Operation”

chapters.

These mainly involve errors in inserting con-

trasting equipment, maladjustment of light rings

or the wrong condenser height setting.

n. b.:

Call the Technical Service!

The integrated transmitted light lamp does not

We described all these possible errors in pre- respond.

vious chapters.

● Make sure the plug of the lamp cable is firmly

plugged into the corresponding socket on the

back of the DM IRB stand.

Therefore, if you are not satisfied with the

quality of the image, read the relevant sections

of the manual.

● The halogen lamp may be faulty.

105

Download from Www.Somanuals.com. All Manuals Search And Download.

Replacing the 12 V/100 W halogen lamp

● Insert the new lamp as far as it will go into the

sockets of the lamp holder.

● Mount the lamphousing and screw down with

a 3 mm Allen key.

n. b.:

● Reconnect the transmitted light illumination

column to the power supply on the back of the

stand.

● Connect the microscope and, if used, the

power unit to the mains.

Remember to disconnect from the mains!

Leave the protective cover on until the lamp

is inserted. Avoid making fingermarks, or

wipe off immediately.

The additional fluorescence lamp does not

respond.

● Switch off the microscope and the power unit

(if used).

● Make sure the cable connections lamp – pow-

er unit – mains are correct and complete.

Possible causes for the failure of the

fluorescence lamp are: a defect fuse of the

power unit, a defect lamp or a defect burner in

the lamphousing.

● Disconnect the appliance cable of the

microscope and the power unit.

● Disconnect the transmitted light illumination

column from the power supply on the back of

the microscope.

● Screw off the lamphousing with a 3 mm Allen

key.

● Remove the faulty lamp.

Fig. 84 Lamphousing for transmitted light illumination (cover

removed)

1 Lamp holder (pin base) with 12 V 100 W halogen lamp,

2 Collector, 3 Heat protection filter

Fig. 83 Lamphousing for transmitted light illumination

1 Cover, 2 Cover screws

106

Download from Www.Somanuals.com. All Manuals Search And Download.

Replacing the mains fuse on the power unit*

n. b.:

Remember to disconnect from the mains!

● Switch off the microscope and the power unit.

● Disconnect the appliance cable of the micro-

scope and the power unit.

● Remove the defect fuse from the fuse holder.

Replacement fuses of IEC 127-2 standard

and/or UL 198 G and/or company type:

Fig. 85 Lamphousing 107/2

1 Screw for opening the lamphousing

Part no.:

Name:

846-205.000-00

T 4 A

Wickmann 19 195/

Schutter FST

Fig. 87 Lamphousing 106, opened

Fig. 86 Lamphousing 107/2, opened

1 Collector, 2 Holder with 12 V 100 W halogen lamp

1 Screw for opening the lamphousing, 2 Holder with 12 V

100 W halogen lamp, 3 Collector, 4 Diffusing disc

107

Download from Www.Somanuals.com. All Manuals Search And Download.

n. b.:

Never use fuses with a different rating from the

ones specified.

Always disconnect external transformers

and the microscope from the mains when

carrying out assembly work!

● Connect the microscope and the power unit to

the mains.

● Switch off the microscope and the power unit.

● Disconnect the appliance cable of the micro-

scope and the power unit.

● Slacken the clamp screw on the microscope

and remove the lamphousing.

● Slacken the screw (85.1) on the lid and re-

move the lid.

● Move the collector (84.3) to the front if neces-

sary.

Replacing the 12 V / 100 W halogen lamp

in lamphousing 106, 107, 107/2

Ask a member of Leica field staff to show you

how to change the halogen lamp properly.

Here again are all the necessary steps:

n. b.:

This step is not necessary with lamphousing

107/2.

Fig. 88 Lamphousing 106 z, opened

1 Lid, flipped up, 2 Collector, 3 12 V 100 W halogen or gas

discharge lamp, 4, 9 Screw holes for lid, 5 Reflector, 6, 8 x-y

adjustment screws for centration of reflector, 7 Reflector

focusing, 10 Fixing screws for lamp holder, 11 Socket for cut-

out plug

Fig. 89 12 V 100 W lamp holder

108

Download from Www.Somanuals.com. All Manuals Search And Download.

● Switch off the microscope and the power unit.

● Disconnect the appliance cable of the micro-

scope and the power unit.

n. b.:

● Slacken the clamp screw on the microscope

and remove the lamphousing.

● Slacken the screws (88.4 and 88.9) on the lid

with a cross-tip screwdriver.

Leave the protective cover on until the lamp

is inserted. Avoid making fingermarks, or

wipe off immediately.

● Pull the cut-out plug slightly out of the socket

(88.11) and flip up lid.

● Remove the defect lamp.

● Put a new 12 V 100 W halogen lamp into the

lamp holder without tilting (86.2 or 87.2).

● Move the collector back.

● Put on the lid and secure with screw (85.1 or

87.1).

● Align the lamphousing against the microscope

and secure with the clamp screw.

● Connect the lamphousing to the power unit.

n. b.:

Leave the protective cover on until the lamp

is inserted. Avoid making fingermarks, or

wipe off immediately.

Replacing the 12 V 100 W halogen lamp in

lamphousing 106 z*

● Slacken the fixing screws (88.10) on the lamp

holder and pull out the lamp holder (Fig. 89).

● Remove the defect lamp.

● Put a new 12 V/100 W lamp into the lamp

holder.

n. b.:

● Push in the lamp holder and secure it with the

screws (88.10).

● Push the cut-out plug into the socket (88.11).

● Close the lid and tighten the screws (88.4 and

88.9) on the lid.

Always disconnect external transformers

and the microscope from the mains when

carrying out assembly work!

● Align the lamphousing against the microscope

and secure with the clamp screw.

● Connect the lamphousing to the power unit.

109

Download from Www.Somanuals.com. All Manuals Search And Download.

Changing the Hg and Xe lamps

on lamphousing 106 z

● Disconnect the power unit and the micro-

scope from the mains.

● The LH 106 z L is opened by undoing the fixing

screws (88.4), pulling the cut-out plug slightly

out the socket (88.11) and flipping up the lid of

the lamphousing.

n. b.:

● Slacken the safety screws (88.10) and pull out

the lamp holder (Fig. 90).

● Insert the burner as follows, making abso-

lutely sure to observe the safety measures

described above:

● Always disconnect the power unit from the

mains before carrying out assembly work.

● Wait for the lamphousing to cool down for

at least 15 minutes as otherwise it may

explode.

● If there is a plastic cover on the burner, leave

it on for the time being.

● Never touch glass parts of the burner with

your bare hands as finger perspiration

burns in.

● Wipe off any finger perspiration and dirt

carefully (perhaps using alcohol).

● Adjust the lamps immediately after ignition.

● Avoid switching on and off frequently, as

this greatly reduces the life and stability of

the lamp. Hot Hg lamps do not ignite again

until they have cooled down. It is advisable

to let new burners burn in for a few hours

without interruption.

● Ensure that lamphousing is adequately

ventilated. Never block the air vents with

paper, etc. (fire risk).

● Insert the burner so that the lettering is

upright after insertion (different diameters of

the metal base for the Hg 100 and Xe 75

burners ensure that these are always inserted

the right way up).

● If the bulb has a glass seal point (90.2), the

burner is turned so that this point will be at the

side, not in the light path.

● Put the upper pin of the burner between the

clamps of the flexible power supply and clamp

with screw (90.1).

● Unscrew the stud (90.4) in the holder slightly,

insert the lower end of the metal base and

retighten the stud.

● It is best to keep a record of the number of

hours a lamp has been in use and compare

it with the manufacturer’s specifications.

● We cannot accept any liability for damage

resulting from lamp explosions.

● Remove the protective covering from the

burner now.

110

Download from Www.Somanuals.com. All Manuals Search And Download.

● Put the lamp holder with burner inserted into ● Push the cut-out plug in as far as it will go.

the lamphousing and secure with the screws ● Align the lamphousing against the microscope

(90.10).

and secure with the clamp screw.

● Close the lid of the lamphousing. When ● Connect the lamphousing to the power unit

closing the lamphousing, make sure that the

pins of the cut-out plug engage in the sockets.

● Retighten the screws of the lid.

(compare mains voltage!).

Fig. 90 Lamp holders for gas discharge lamps

1 Upper clamp, 2 Seal point of the burner, 3 Lower clamp, 4, 6 Drill holes for fixing the lamp holder, 5 Sockets for cut-out plug,

7 Protective cover

Xe 75

Hg 50

Hg 100

Stab.

111

Download from Www.Somanuals.com. All Manuals Search And Download.

Storage

Packaging and

transport

Protect your microscope from dust by putting on The original packaging should be used if the

the cover after each work session.

microscope has to be dispatched or trans-

ported. Also, the delivery note with full details

The microscope must be kept in a cupboard in should be enclosed.

which the temperature is ≥ 5°C above room

temperature. The cupboard must have ven-

tilation holes which are plugged with cotton

wool, for example, to keep dust out. If this type

of storage is not possible, the microscope is

kept in a closed container with drying agent

(e. g. silicone balls).

112

Download from Www.Somanuals.com. All Manuals Search And Download.

Technical description

All techniques, not only in microscopy, are Objective lettering

subject to limits of performance due to basic

Examples and explanation of symbols:

physical laws and principles of eye physiology.

The following information should therefore be

remembered when using the microscope.

∞ / -

C PLAN 10x/0.22

∞ / 0.17

Performance data of objectives

C PLAN 40x/0.65

The Leica DM IRB microscope is based on tube

length ∞ (infinity) and a focal length of the tube

lens of f = 200 mm.

∞

Objective for tube length infinity (∞).

n. b.:

–

Therefore, only objectives with the ∞ engraving

The objective can be used with and without a

coverslip.

and M 25 screw thread may be used.

The current objective range is constantly being

updated. Please ask your Leica agency for a

copy of the “Objective data sheets”!

0 – 2

For use with coverslips with a thickness of

0 – 2 mm.

0.1 – 1.3

For use with coverslips with a thickness of

0.1 – 1.3 mm.

0.17

The objective may only be used with a coverslip

of the standard thickness 0.17 mm. No coverslip

or a coverslip with a very different thickness will

greatly impair the image, particularly with high

objective apertures (see below).

Use without a coverslip, e. g. for cell smears,

incident light.

113

Download from Www.Somanuals.com. All Manuals Search And Download.

D (or A, B, C)

10x/0.22

Magnification and aperture. The aperture

(pick-up angle) influences resolution, field

depth, contrast and brightness. Objectives with

built-in iris diaphragm have an engraving

showing the maximum and minimum aperture,

e. g. 0.85 – 0.55.

Pupil position of objective (important, e. g. for

IMC integrated modulation contrast).

Objective type (performance class):

C Plan

Achromatic objective with particularly good

price/performance ratio. Field performance

max. 20 mm.

n. b.:

Objectives with built-in iris diaphragm.

The knurled ring may only be used for adjusting

the diaphragm, not for screwing the objective in

or out.

N Plan

Achromatic objective with increased field per-

formance of at least 20 mm.

Risk of damge!

PL FLUOTAR ^®

Semiapochromats with particularly good field

performance of at least 25 mm and chromatic

correction. Universal optics for all techniques.

OIL, W, IMM

Immersion objectives for oil, water, universal

(oil, glycerine, water, etc.).

PL APO

PH = phase contrast objective, with additional

indication of assigned light ring in condenser,

e. g. PH2.

Plan apochromats with a field performance of

over 25 mm and maximum chromatic correction.

The best objectives in the Leica range.

BD = brightfield/darkfield; objectives for incident

light microscopy with M 32 screw thread.

Harmonic Components.

CORR

Universally applicable, also backwards com-

patible with Delta optics (= predecessors of HC

optics).

Correction mount for continuous adjustment to

coverslip/specimen slides or thickness of vessel

base.

P, POL

Long working distance.

Strain-free objective for quantitative polarisa-

tion microscopy.

U-V-I

With special achromatic correction, i.e. parfocal

from the ultraviolet through the visual to the

near infrared range (from 340 nm to 1000 nm).

114

Download from Www.Somanuals.com. All Manuals Search And Download.

Colour coding of the objectives

The magnification of each objective is indicated as per DIN/ISO standard by a colour ring:

100x

125x

150x

160x

63x

40x

50x

25x

32x

16x

20x

10x

6.3x

2.5x

1.6x

white

dark

blue

light

blue

dark

green

light

green

yellow orange red

brown

grey

Immersion objectives also have a second colour ring underneath:

Black

Oil or IMM (= universal for oil,

water, glycerine)

Water

The different colour of the objective engraving

indicates the use of the objective:

White

Orange

Black or

dark blue

Green

Brightfield objectives,

low-strain

Phase contrast objectives,

low-strain

Glycerine

Locking of objectives

The front part of immersion objectives (OIL, W,

IMM) can be pushed up (91.1 and 91.2) by about

2 mm and locked in a shortened position by a

slight rotary movement. This stops any

remaining drops of immersion liquid from

wetting specimens or other objectives when the

nosepiece is turned.

Fig. 91 Immersion objectives

1, 2 Oil immersion objectives (OIL), 1 in working position,

locked in shortened position, 3 Water immersion objective

(W), Universal immersion objective (IMM) for water,

glycerine, oil, 5 Colour coding for immersion, 6 Knurled ring

for screwing down

115

Download from Www.Somanuals.com. All Manuals Search And Download.

Performance data of eyepieces

Leica eyepiece

type

Magnification/ Eyepiece port +)

fov

The maximum eyepiece field of view of a

specific configuration is derived from the

following microscope data:

HC PLAN

10x/20

12.5x/16

10x/20

10x/22

11x/20

Field performance of the objectives

Field performance of the intermediate

module(s)

Eyepiece tube diameter: 30 mm

Tube field number

Condenser properties

Withremovableorpush-backanti-glareprotection

for use with or without eyeglasses.

Ajustable eyelens (dioptre compensation) and slot

for graticules of 26 mm diameter.

The decisive value is always the smallest.

For example, if the intermediate modules only

permit a field of view of 20 mm, but the

objectives and tube 25 mm, only eyepieces up to

fov 20 can be used. Eyepieces with fov 25 can

lead to vignetting in this case.

With illuminated graticule.

The LEITZ PERIPLAN^®eyepiece type may not be used! Earlier

L PLAN type eypieces may only be used with earlier type tubes

(before about 1988) without the HC engraving!

The diameter of the viewable specimen area is

calculated by dividing the diameter of the field

of view by the magnification of the objective and

the magnification factor of the microscope

optics.

Eyepiece field of view

Each microscope configuration has a certain

eyepiece field of view (see below), e. g. 20,

which must not be exceeded. If the maximum

fov is exceeded there may be disturbing loss of

definition and/or vignetting at the edge of the

image, → following pages!

Example:

Eyepiece 10x/20

Objective PLAN 4/0.10

Magnification factor of the Leica DM IRB

Microscope optics 1x

The eyepiece field of view (fov) designates the

diameter of the intermediate image in the

eyepiece in mm, i.e. the diameter of the circular

diaphragm that frames the image and that lies

inside the eyepiece. This fov is specified on the

eyepiece after the magnification, e. g. 10x/20.

For the Leica DM IRB microscope we recom-

mend fov 22.

Viewable specimen area

20 mm

––––––––– = Ø 5 mm

4 x 1

The total magnification of the microscope is

worked out by multiplying the eyepiece mag-

nification with the reproduction ratio of the

objective and the magnification factor of the

microscope optics.

116

Download from Www.Somanuals.com. All Manuals Search And Download.

Example:

Your Leica agency can supply you with a

constantly updated data sheet on all Leica

objectives.

Eyepiece 10x/20

Objective PLAN 4/0.10

Magnification factor 1x

Eyepiece graticules

Total magnification 10 x 4 x 1 = 40x

Graticules for length measurements and grain

and particle measurements

Field performance of objectives

The field performance of objectives is not Our product range comprises the following

engraved on the objectives. It may vary within graticules:

the same class, e. g. low objective magnifi- ● Graticule

cations may well exhibit slightly higher values

than the average values given below:

10 mm/100 divisions

● Graticule 10 mm/100 divisions

with crosshair

● Graticule for standard series

and Snyder-Graff method

● Graticule ASTM-E-112,

grain size determination

● Graticule with 10 x10 x 0.1 mm

grid divisions

Order no. 506 950

Order no. 506 952

Order no. 566 950

Order no. 566 951

Order no. 506 954

Order no. 506 955

Objective series

max. recommended

eyepiece fov

15 20 22

Achromats

C PLAN achromats

APO L apochromats

N PLAN planachromats

PL FLUOTAR^®semiapochromats

PL APO planapochromats

● Graticule with 10 x10 x1 mm

grid divisions

For calibrating the graticules, we recommend:

Incident light stage micrometer

1 mm = 100 divisions

Order no. 563 011

Fig. 92 Optical outfit

Fig. 93 Eyepiece 16x/14B

1 N PLAN objective series for brightfield, 2 HC PLAN 10x/20

and , HC PLAN 10x/22 and M eyepieces

1 Clamp screw, 2 Spacer rings for Leica microscopes (must

be pushed up as far as the stop)

117

Download from Www.Somanuals.com. All Manuals Search And Download.

Filter performance data

Filter

Use

Grey/neutral density filter N

Grey (neutral density) filters are used for light

attenuation without influencing the colour

temperature. The engraved value, e. g. N16,

indicates the attenuation value. So N16 means a

reduction to 1/16 = 100/16 = 6.25 % transmission.

Green filter, GR, panchromatic

For general contrast enhancement and black-

and-white photography.

DLF

Conversion filter (Daylight filter, blue, similar to

CB12), for colour photography with daylight film,

integrated in filter magazine.

BG38 (blue filter)

Suppresses red for fluorescence (integrated in

fluorescence illuminator).

ALF

Artificial light filter for enhancement of colour

contrast in colour photography with artificial

light films.

BG20

For highlighting red in Polaroid colour photo-

graphy.

VG9 (green filter)

Enhances contrast in chromosome photography.

CB1.5, CB3

Conversion filter, blue: raises colour tempera-

ture with special lamps.

CR1.5

BG23

Conversion filter, red: lowers colour tempera-

ture, e. g. from 6000 K (colour temperature of an

Xe lamp) to 5500 K (colour temperature of

daylight film).

Enhances contrast of the complementary col-

ours blue and red for black-and-white film.

118

Download from Www.Somanuals.com. All Manuals Search And Download.

Tube performance data

Binocular tube HCI B22

Tube changing is the same as for the upright The binocular tube consists of a basic part with

microscopes.

The tubes are interchangeable.

the tube change ring at the bottom. The tube

lens has the factor 1x. The Siedentopf binocular

part allows adjustment of the interpupillary

distance from 55 mm to 75 mm. The viewing an-

gle is 45°. The tube has adjustable eyepiece

tubes for mechanical compensation of the

tube length when the interpupillary distance

changes. It allows a field of view index of 22.

Fig. 94 HCI B22, Binocular tube with 45° viewing angle, field

of view index up to 22, eyepiece diameter 30 mm for HC PLAN

10x/20 or 22 eyepieces, interpupillary distance setting:

55 – 75 mm

1 Clamp screw, 2 Tube port, 3 Siedentopf binocular part

Binocular ergotube HCI BV22

Like HCI B22, but with variable viewing angle of

15° – 30°.

Fig. 95 HCI 3T22, Trinocular tube, 45° viewing angle,

Light path: 100 % vis

– switch rod

150 % – 50 % – switch rod

100 % – photo – switch rod

Field of view index up to 22, eyepiece diameter 30 mm for HC

PLAN 10x/20 or 22 eyepieces, interpupillary distance setting:

55 – 75 mm

1 Clamp screw, 2 Tube port, 3 Siedentopf binocular part,

4 Photo/TV exit, 5 Switch rod

Fig. 96 HCI BV22, ergo binocular tube with 15° – 50° viewing

angle, field of view index up to 22, eyepiece diameter 30 mm

for HC PLAN 10x/20 or 22 eyepieces, interpupillary distance

setting: 55 – 75 mm

1 Clamp screw, 2 Tube port, 3 Siedentopf binocular part

119

Download from Www.Somanuals.com. All Manuals Search And Download.

Trinocular tube HCI 3T22

HC FSA 25 PR

The trinocular tube consists of a basic part with Binocular observation and photo tube, viewing

the tube change ring at the bottom, the tube angle 30°, with back reflection.

lens has the factor 1x. The Siedentopf binocular Controllable dark flap of the binocular port for

part allows adjustment of the interpupillary photography and microphotometry.

distance from 55 mm to 75 mm. The viewing an-

gle is 45°. The tube has adjustable eyepiece 3 clickstop positions of the beamsplitter in the

tubes for mechanical compensation of the tube tube:

length when the interpupillary distance

changes. It allows a field of view index of 22. Switch rod

The documentation port is only operated with VIS

Observation

100 %

Photo

0 %

HC components.

The tube contains a switchable mirror with PHOTO

three settings:

50/50

150 %

110 %

50 %

100 %

visual

Back reflection only at the 50 % / 50 % beam-

splitter position.

150 % / 50 % visual/photo

100 %

photo

Tube adapter R/IR

The tube adapter R/IR enables compatibility of

all tubes with viewing angle 30° of the Leica

DM R range.

On the Leica DM IRB/E these tubes are only

suitable up to 22 fov.

120

Download from Www.Somanuals.com. All Manuals Search And Download.

HC FSA 25 PE

Condenser performance data

Binocular observation and photo tube, viewing Condenser 0.30 S70

angle 30°, with provision for optical overlay for

Without height adjustment, as the fixed focus

documentation of transparencies (slide overlay

device) or opaque macro objects (macro de-

vice).

concept of this condenser guarantees optimal

matching of light and phase rings for liquid

levels up to 60 mm. FWD (free working distance)

70 mm. For brightfield (HF), phase contrast (PH,

Phaco), transmitted light interference contrast

(ICT) and polarisation contrast up to 40x ob-

jective magnification in each case.

3 clickstop positions of the beamsplitter in the

tube:

Switch rod

VIS

Observation

100 %

Photo

0 %

Condensers 0.53 S23 and 0.90 S1

50/50

PHOTO

150 %

110 %

50 %

100 %

The condenser has a slide changer, height

adjustment and centration facility for setting

Koehler illumination. The holder holds the base

part of the condenser, which can be fitted with

condenser tops 0.53 S23, 0.90 S1 or P 1.40 OIL S1

to suit the particular application.

Possible applications of the condensers for the Leica DM IRB

Illumination

technique

Condenser

0.30 S70 objective accessories

Diaphragms/

Condenser

0.53 S23 objective accessories

Diaphragms/

Condenser

0.90 S1 objective accessories

Diaphragms/

Brightfield

Phaco

2.5x – 40x

–

5x – 100x

–

10x – 100x ●

–

10x, 20x

40x

–

PH 0 S70

PH 1 S70

PH 2 S70

–

PH 0 S23

PH 1 S23

PH 2 S23

PH 3 S23

–

10x, 20x

40x, 63x

100x

10x, 20x

40x, 63x

100x

PH 1 S1

PH 2 S2

PH 3 S3

Interference

contrast

ICT

device

ICT

device

ICT

device

࠘

10x – 40x

2.5x – 40x

–

10x – 100x

5x – 100x

10x – 100x

Pol contrast

Darkfield

Pol device

–

Pol device

3 S23

10x – 100x ●

Pol device

D S1

5x – n. a. 0.40

10x – n. a. 0.75

࠘

100 x for maximum resolution also possible with condenser top P 1.40 OIL S1

Light ring 3 S23 serves as DF diaphragm

For maximum resolution there is also a condenser top P 1.40 OIL S1

●

121

Download from Www.Somanuals.com. All Manuals Search And Download.

Together with condenser top 0.53 S23, max. Performance data of stages

FWD 30 mm, culture vessels can be examined and mountable object guides

microscopically up to liquid levels of 25 mm. For

contrasting techniques brightfield (HF), phase Plane stage

contrast (Phaco/PH), transmitted light inter-

With hole for insert rings of 20 mm diameter or

ference contrast (ICT) and polarisation contrast,

objectives with magnifications up to 100x can

be used. For transmitted light darkfield we

recommend objectives with a numerical aper-

ture up to 0.40.

40 mm diameter. Holes for inserting specimen

clips and two threaded holes on left and right

underneath the stage for attachment of the

object guide.

For condenser top 0. 90 S1, max. FWD 1 mm, thin

specimen slides and coverslips must be used as

substrates for the specimen. Objectives with a

numerical aperture up to 0.75 are suitable for

transmitted light darkfield. All other contrasting

techniques can be performed up to objective

magnification 100x.

Object guide

Adjustment range: X 127 mm x Y 83 mm

To accommodate holders for different culture

vessels. Self-adhesive scales for the holders are

enclosed for coordinate adjustment reading.

These should be stuck in the countersinks of the

object guide.

Condenser disc

3-plate x/y stage

All condensers of the Leica DM IRB are

equipped with a 6-position disc which can be

fitted with an individual choice of annular stops

for phase contrast (PH), darkfield (DF) or IC

prisms for transmitted light interference con-

trast (ICT).

Adjustment range: X 60 mm x Y 40 mm

With hole for insert rings of 20 mm diameter or

40 mm diameter. Holes for inserting specimen

clips. Coaxial drive for specimen positioning

with universal joint.

Rotary stage with object guide

and frame insert for coverslips

Rotation radius: 360°

Adjustment range of the object guide:

X 40 mm x Y 40 mm

The object guide accommodates specimen

slides or the frame insert for coverslips. The

minimum size of coverslips that can be secured

in the frame insert is 22 mm x 32 mm.

122

Download from Www.Somanuals.com. All Manuals Search And Download.

Stages

Lamphousing performance data

Lamphousing 106*

Lamphousing 106 is equipped with a 12 V 100 W

Plane stage and mountable object guide

The plane stage is secured to the microscope halogen lamp. The lamp holder is centrable in x

with 3 screws. The object guide can be mounted and y direction. The aspherical collector can be

to either the right or the left of the plane stage.

focused. Lamphousing 106 is fitted with a

diffusing disc and heat protection filter, but does

not have a reflector.

3-plate x/y stage

To attach the stage, 3 screw holes first have to

be accessed by moving the stage in x/y direc- Lamphousing 106 z*

tion.

Like lamphousing 106, but additionally with

centrable and focusable reflector and 4- to 6-

lens collector. A quartz collector is available on

Rotary stage and frame insert for coverslips

The rotary stage is secured with 3 screws. Move request. The following lamps can be used (each

the rotary mount to gain access to all the screw have their own holder):

holes. Put the screws in the holes.

– 12 V/100 W halogen lamp (A.C.)

n. b. Use washers as well for the holes at the – Ultra high pressure 50 W Hg lamp (A.C.)

back. Only screw the screws in lightly, as the – Ultra high pressure 100 W Hg lamp

rotary stage first has to be centred: to do this,

insert the centring aid in the rotary stage. – Ultra high pressure 100 W Hg lamp

Engage the Bertrand lens by turning the knurl (D.C. stabilised/non-stabilised, type 103 W/2)

and focus with the lever. Move the stage until – High pressure 75 W xenon lamp

(D.C. stabilised/non-stabilised)

the bright circle is in the centre of the field of

view. Then fix the stage in position, disengage

(D.C. stabilised/non-stabilised).

the Bertrand lens and remove the centring aid. Lamphousing 107/2

To secure specimen slides in frame inserts, The shield connection of the lamphousing is

press the middle of the leaf spring and slide in screwed to the potential equalisation point of

the coverslip in the direction of the arrow. Clamp the 12 V 100 W power unit. This lamphousing for

the frame insert in the object guide.

transmitted and incident light has a fixed 1-lens

collector and a fixed 12 V 100 W lamp.

Performance data of the incident light

fluorescence illumination*

n. b.

The Leica DM IRB microscope is preferably

equipped with mercury or xenon gas discharge

lamps for fluorescence applications as they

offer higher intensity. However, a 12 V 100 W

halogen lamp can be used as well.

Lamphousings LH 105 have been replaced by

lamphousings LH 106. However, they are

compatible with LH 106 lamphousings and can

also be used.

123

Download from Www.Somanuals.com. All Manuals Search And Download.

Type

Average life span

12 V 100 W halogen lamp (A.C.)

Hg ultra high pressure lamp 50 W (A.C.)

Xe high pressure lamp 75 W (D.C., stabilised)

Hg ultra high pressure lamp 100 W (D.C., stabilised/non-stabilised)

Hg ultra high pressure lamp 100 W (D.C., stabilised/non-stabilised, type 103 W/2)

100 h

400 h

200 h

300 h

Lamphousings with order nos.

Non-centrable lamphousings

LH 106

LH 107, left

504086

LH 107/2

LH 35/2

504088

6 V/35 W

12 V/100 W, 0.55 m

12 V/100 W, 2.0 m

12 V/100 W, 2.0 m,

shielded

504 058

504 059

504 080

504 085

Centrable lamphousings

LH 106, right-hand op.

LH 106, left-hand op.

4-lens

6-lens

12 V/100 W, 0.55 m

12 V/100 W, 2 m

12 V/100 W, 2.9 m

507 070

504 071

504 087

504 065

Hg 100 W, with ZG

Hg 100 W, with ZG, 3 m

Hg 100 W, without ZG

Hg 50 W

504 068

504 069

504 083

504 062

504 063

504 090

504 066

504 089

Xe 75 W

504 061

124

Download from Www.Somanuals.com. All Manuals Search And Download.

General technical data

For indoor use only

Mains voltage:

90 – 250 V ~

Frequency:

50 – 60 Hz

Power consumption:

Fuses:

DM IRB max. 160 W

T 4 A

Ambient temperature:

Relative humidity:

Overvoltage category:

Contamination class:

10 – 36 °C

0 – 80 % up to 30 °C

125

Download from Www.Somanuals.com. All Manuals Search And Download.

Technical data of the power unit

General technical data

For indoor use only

Mains voltage:

90 – 250 V~

Frequency:

50 – 60 Hz

Power consumption:

Fuses:

160 W

T 4 A

Ambient temperature:

Relative humidity:

Overvoltage category:

Contamination class:

10 – 36 °C

0 – 80 % up to 30 °C

Technical specifications

Lamp voltage:

Adjustable from

2.5 V ± 5 % to 12 V – 5 % /8.5 A

Voltage setting:

Potentiometer 5 KOhm

Rotated clockwise for maximum intensity

Maximum lamp voltage:

Soft start:

12.0 V in the range 90 V to 250 V~

Rise time up to

maximum output voltage 0.2 to 1 second

Mains voltage dependence

U_N= mains voltage

U_La= lamp voltage

U_N: 90 – 250 Vac, U_La= 12 V:

U_N: 90 – 250 Vac, U_La< = 11 V:

U_N: 100 – 130 Vac, U_La< = 11 V:

U_N: 200 – 250 Vac, U_La< = 11 V:

< – 5 %

< ± 1 %

< ± 0.5 %

Lamp voltage drift 0 to 10 min.:

Efficiency:

< 2 %

approx. 75 %

Short-circuit and open-circuit proof

Life span:

> 50,000 hours

126

Download from Www.Somanuals.com. All Manuals Search And Download.

Main wearing and spare parts, tools

Order No.

Part no.

Component

Used for

Spare lamps

500 974

500 137

500 138

in preparation

Halogen lamp

12 V 100 W

Lamphousing 105

Lamphousing 106 z

Ultra high pressure Hg lamp 50 W

Ultra high pressure Hg lamp 100 W

(103 W/2)

500 139

High pressure xenon lamp 75 W

Lamphousing 106 z

Tools, adjustment keys

016-500.020-001

020-434-045

Hexagonal screwdriver

2.5 mm Allen key,

angled, short

Assembly and adjustment

Assembly of heating stage and

illumination mirror

Screw cover for unoccupied nosepiece positions

020-422.570-000

Screw cover M25

Objective nosepiece

Spare eyecups (glare protection) for HC PLAN eyepiece

021-500.017-005

021-264.520-018

Eyecup HC PLAN

10x/25 eyepiece

10x/22 eyepiece

10x/20 eyepiece

Immersion oil, DIN/ISO standard, fluorescence-free

513 787

513 522

513 788

110 ml

100 ml

500 ml

OIL and IMM objectives

and oil condenser tops

Spare fuses, IEC 127-2 and/or UL 198 G standard and/or company type:

846-205.000-00

T 4 A

Leica 12 V 100 W

Wickmann 19 195/

Schutter FST

127

Download from Www.Somanuals.com. All Manuals Search And Download.

EU Conformity declaration

We hereby declare that the product specified

below conforms in its design and construction

as well as the model we have put on the market

to the relevant safety and health regulations laid

down by the European Union.

This declaration will cease to be valid if the

instrument is modified without our consent.

Product name:

Instrument type:

Instrument no.:

DM IRB

Light microscope

020-525.701 to

020-525.780

EU directives:

Low voltage:

73/23/EWG

Electromagnetic

compatibility:

89/336/EWG

Harmonised

standards

applied:

EN 50081-1

EN 50082-1

EN 61010-1

Wetzlar, 18. 4. 1997

Prof. Dr.-Ing. habil. M. Jaksch,

Director of Technology

and Development Engineering

128

Download from Www.Somanuals.com. All Manuals Search And Download.

Tel. +49 (0) 64 41-29 0

Fax +49 (0) 64 41-29 25 99

www.leica.com

Leica Microsystems Wetzlar GmbH

Ernst-Leitz-Straße

D-35578 Wetzlar (Germany)

Download from Www.Somanuals.com. All Manuals Search And Download.

Liebert Network Card ITR User Manual
Lightolier Work Light 22OR2 User Manual
Little Wonder Vacuum Cleaner Little Wonder User Manual
LOREX Technology TV Cables CVA6950 User Manual
Magnavox TV VCR Combo CCB130CN User Manual
Marshall electronic Flat Panel Television V R84DP 2C User Manual
Maytag Clothes Dryer MD98 User Manual
Maytag Refrigerator MFI2266AEW User Manual
McCulloch Lawn Mower MM4756SMD User Manual
Mercedes Benz Barcode Reader CS1000 User Manual